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Purified Mouse Anti-IRF1
Purified Mouse Anti-IRF1

Western blot analysis of IRF1 on a MOLT-3 cell lysate (Human T-lymphoblasts; ATCC CRL-1552). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-IRF1 antibody.

Western blot analysis of IRF1 on a MOLT-3 cell lysate (Human T-lymphoblasts; ATCC CRL-1552). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-IRF1 antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human IRF1 aa. 159-279
Western blot (Routinely Tested), Flow cytometry (Tested During Development), Immunofluorescence (Not Recommended)
48 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
612046 Rev. 2
Antibody Details
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The 20/IRF-1 monoclonal antibody specifically binds to Interferon regulatory factor 1 (IRF1). IRF1 belongs to the human IRF family of transcription factors that contains nine members, IRF1-9. IRF1 is broadly expressed and its levels are upregulated in response to certain cytokines, interferons, bacterial or viral infections. IRF1 binds to specific DNA motifs such as IFN-stimulated response elements (ISRE) and regulates the activity of IFN-responsive genes. IRF1 plays roles in inflammation as well as innate and adaptive immune responses. It is involved in the regulation of cellular proliferation, Major Histocompatibility Complex (MHC) antigen expression, and the differentiation and effector functions of macrophages, dendritic cells, natural killer (NK) cells, and various T cell subsets. In addition, IRF1 may function as a tumor suppressor, since deletions in the IRF1 locus is associated with myelodysplasia and leukemias.

612046 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612046 Rev.2
Citations & References
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Development References (8)

  1. Cha Y, Sims SH, Romine MF, Kaufmann M, Deisseroth AB. Human interferon regulatory factor 1: intron-exon organization. DNA Cell Biol. 1992; 11(8):605-611. (Biology). View Reference
  2. Ikushima H, Negishi H, Taniguchi T. The IRF family transcription factors at the interface of innate and adaptive immune responses.. Cold Spring Harb Symp Quant Biol. 2013; 78:105-16. (Biology). View Reference
  3. Kim SK, Fouts AE, Boothroyd JC. Toxoplasma gondii dysregulates IFN-gamma-inducible gene expression in human fibroblasts: insights from a genome-wide transcriptional profiling.. J Immunol. 2007; 178(8):5154-65. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
  4. Kroger A, Ortmann D, Krohne TU, et al. Growth suppression of the hepatocellular carcinoma cell line Hepa1-6 by an activatable interferon regulatory factor-1 in mice. Cancer Res. 2001; 61(6):2609-2617. (Biology). View Reference
  5. Romeo G, Fiorucci G, Chiantore MV, Percario ZA, Vannucchi S, Affabris E. IRF-1 as a negative regulator of cell proliferation.. J Interferon Cytokine Res. 2002; 22(1):39-47. (Biology). View Reference
  6. Savitsky D, Tamura T, Yanai H, Taniguchi T. Regulation of immunity and oncogenesis by the IRF transcription factor family.. Cancer Immunol Immunother. 2010; 59(4):489-510. (Biology). View Reference
  7. Varley CL, Bacon EJ, Holder JC, Southgate J. FOXA1 and IRF-1 intermediary transcriptional regulators of PPARgamma-induced urothelial cytodifferentiation.. Cell Death Differ. 2009; 16(1):103-14. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  8. Xie R, van Wijnen AJ, van Der Meijden C, Luong MX, Stein JL, Stein GS. The cell cycle control element of histone H4 gene transcription is maximally responsive to interferon regulatory factor pairs IRF-1/IRF-3 and IRF-1/IRF-7. J Biol Chem. 2001; 276(21):18624-18632. (Biology). View Reference
View All (8) View Less
612046 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.