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Purified Mouse Anti-Human CD182
Purified Mouse Anti-Human CD182

Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD182 (Cat. No. 555932; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) and secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.

Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD182 (Cat. No. 555932; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) and secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.

Product Details
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BD Pharmingen™
CXCR2; CXCR-2; IL8RB; IL-8Rβ; Interleukin 8 receptor, beta; CDw128b
Human (QC Testing)
Mouse IgG1, λ
Human CXCR2 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
3579
AB_396229
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555932 Rev. 8
Antibody Details
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6C6

The 6C6 monoclonal antibody specifically binds to CD182. CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8. IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes.

555932 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555932 Rev.8
Citations & References
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Development References (5)

  1. Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol. 1995; 17(2):103-108. (Biology). View Reference
  2. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  3. Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
  4. Qin S, LaRosa G, Campbell JJ, et al. Expression of monocyte chemoattractant protein-1 and interleukin-8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential. Eur J Immunol. 1996; 26(3):640-647. (Biology). View Reference
  5. Wu L, Ruffing N, Shi X, et al. Discrete steps in binding and signaling of interleukin-8 with its receptor.. J Biol Chem. 1996; 271(49):31202-9. (Biology). View Reference
View All (5) View Less
555932 Rev. 8

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.