Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with PerCP-Cy™5.5 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 552093) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; Left Panel) or PE Rat Anti-Mouse CD63 antibody (Cat. No. 564222; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
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Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with PerCP-Cy™5.5 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 552093) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; Left Panel) or PE Rat Anti-Mouse CD63 antibody (Cat. No. 564222; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
Cd63; Tspan30; Melanoma 1 antigen; ME491; C75951
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Mouse Eosinophils
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
0.2 mg/ml
AB_2738678
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554689
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PerCP-Cy™5.5 Rat Anti-Mouse Ly-6G and Ly-6C RUO
Size
0.1 mg
Cat No.
552093
564222 Rev. 2
Antibody Details
NVG-2
The NVG-2 monoclonal antibody specifically binds to CD63 which is also known as Tspan30, Melanoma 1 antigen, ME491, and C75951. CD63 is a tetraspanin which belongs to the transmembrane-4 glycoprotein superfamily (TM4SF). It constitutes a major component of lysosomal membranes and translocates to the cell surface in response to various stimuli. CD63 is expressed by activated platelets, granulocytes, monocytes, macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 can associate with other membrane molecules and functions in cellular signaling, adhesion and motility.
564222 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564222 Rev.2
Citations & References
View product citations for antibody "564222" on CiteAb
Development References (2)
-
Miyamoto H, Homma M, Hotta H. Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages. Biochim Biophys Acta. 1994; 1217(3):312-316. (Biology). View Reference
-
Verjan Garcia N, Umemoto E, Saito Y, et al. SIRPalpha/CD172a regulates eosinophil homeostasis. J Immunol. 2011; 187(5):2268-2277. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunofluorescence). View Reference
564222 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.