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PE Mouse anti-Mouse Ly-108
PE Mouse anti-Mouse Ly-108

Multicolor flow cytometric analysis of Ly-108 expression on mouse bone marrow cells and thymocytes. Mouse bone marrow cells and thymocytes were stained with PE Mouse anti-Mouse Ly-108, Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683) and BD Horizon™ V450 Hamster Anti-Mouse CD3e (Cat. No. 560801) antibodies. Two-color flow cytometric dot plots show the correlated expression of CD45R (bone marrow cells, Left Panel) or CD3 (thymocytes, Right Panel) versus Ly-108 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of Ly-108 expression on mouse bone marrow cells and thymocytes. Mouse bone marrow cells and thymocytes were stained with PE Mouse anti-Mouse Ly-108, Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683) and BD Horizon™ V450 Hamster Anti-Mouse CD3e (Cat. No. 560801) antibodies. Two-color flow cytometric dot plots show the correlated expression of CD45R (bone marrow cells, Left Panel) or CD3 (thymocytes, Right Panel) versus Ly-108 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
CD352; Slamf6; SLAM family member 6; KAL1; NTB-A; SF2000
Mouse (QC Testing)
Mouse IgG2a, κ
WT thymocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
30925
AB_10713170
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561540 Rev. 1
Antibody Details
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13G3

The 13G3 monoclonal antibody specifically binds to Lymphocyte antigen 108, Ly-108. Ly-108 is a member of the signaling lymphocytic activation molecule (SLAM) family of immune receptors. Ly-108 is a type 1 transmembrane glycoprotein adhesion receptor that is expressed by T cells, NKT cells, B cells, NK cells, macrophages, dendritic cells and neutrophils. Ly-108 plays multiple roles in innate and adaptive immunity including costimulation of NK cell cytotoxicity and T cell cytokine responses. Moreover, Ly-108 has been implicated in autoimmunity.

    

561540 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561540 Rev.1
Citations & References
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Development References (4)

  1. Griewank K, C Borowski, Rietdijk S, et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity. 2007; 27(5):751-762. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
  2. Howie D, Laroux FS, Morra M, et al. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005; 10(174):5931-5935. (Biology). View Reference
  3. Li W, Sofi MH, Rietdijk S, Wang N, et al. The SLAM-Associated Protein (SAP)/Fyn/PKCθ Pathway is Required for Thymocyte-mediated CD4 T Cell Development. Immunity. 2007; 27(2):763-774. (Biology). View Reference
  4. Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. J Immunol. 2000; 52(1-2):63-72. (Biology). View Reference
View All (4) View Less
561540 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.