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PE Mouse Anti-Human Podoplanin
PE Mouse Anti-Human Podoplanin

Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line histogram) or PE Mouse Anti-Human Podoplanin (Cat. No. 566793, solid line histogram) at 1 µg/test. The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cytometer System and FlowJosoftware. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line histogram) or PE Mouse Anti-Human Podoplanin (Cat. No. 566793, solid line histogram) at 1 µg/test. The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cytometer System and FlowJosoftware. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
PDPN; T1-alpha; T1A; GP36; GP40; OTS8; T1A2; TI1A; T1A-2; AGGRUS; PA2.26
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Podoplanin Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869871
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566793 Rev. 1
Antibody Details
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LpMab-23

The LpMab-23 monoclonal antibody specifically recognizes a cancer-specific form of human podoplanin. Podoplanin is a 38-44 kDa type I transmembrane glycoprotein that is encoded by PDPN. This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. Although the podoplanin protein is expressed on a wide variety of cell types in normal tissues (including placenta, lung, skeletal muscle and brain), LpMab-23 recognizes an altered glycosylation pattern that occurs on oral cancer cells and it shows minimal reactivity with the surrounding non-cancerous tissue. In contrast, the LpMab-17 monoclonal antibody recognizes a non-glycosylated epitope of podoplanin on normal and cancer cells. Podoplanin induces platelet aggregation via its three platelet aggregation-stimulating (PLAG) domains; it binds C-type lectin domain family 1 member B (Clec1B, also known as CLEC2); and it is involved in actin cytoskeleton organization and in cellular adhesion and migration. It also plays roles in organogenesis, vascular development, inflammatory diseases, tumorigenesis, and cancer cell motility and metastasis.

566793 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566793 Rev.1
Citations & References
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Development References (8)

  1. Astarita JL, Acton SE, Turley SJ. Podoplanin: emerging functions in development, the immune system, and cancer.. Front Immunol. 2012; 3:283. (Biology). View Reference
  2. Chang JE, Turley SJ. Stromal infrastructure of the lymph node and coordination of immunity. Trends Immunol.. 2015; 36(1):30-9. (Biology). View Reference
  3. Dang Q, Liu J, Li J, Sun Y. Podoplanin: a novel regulator of tumor invasion and metastasis. Med Oncol. 2014; 31(9):24. (Biology). View Reference
  4. Miyazaki A, Nakai H, Sonoda T, et al. LpMab-23-recognizing cancer-type podoplanin is a novel predictor for a poor prognosis of early stage tongue cancer. Oncotarget. 2018; 9(30):21156-21165. (Clone-specific: Immunohistochemistry). View Reference
  5. Pan Y, Xia L. Emerging roles of podoplanin in vascular development and homeostasis. Front Med. 2015; 9(4):421-30. (Biology). View Reference
  6. Retzbach EP, Sheehan SA, Nevel EM, et al. Podoplanin emerges as a functionally relevant oral cancer biomarker and therapeutic target. j.oraloncology. 2018; 78:126-136. (Clone-specific: Immunohistochemistry). View Reference
  7. Ugorski M, Dziegiel P, Suchanski J. Podoplanin - a small glycoprotein with many faces. Am J Cancer Res. 2016; 6(2):370-86. (Biology). View Reference
  8. Yamada S, Ogasawara S, Kaneko MK, Kato Y. LpMab-23: A Cancer-Specific Monoclonal Antibody Against Human Podoplanin. Monoclon Antib Immunodiagn Immunother. 2017; 36(2):72-76. (Immunogen: Flow cytometry, Immunohistochemistry). View Reference
View All (8) View Less
566793 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.