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PE Mouse Anti-Human IL-4
Product Details
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BD Pharmingen™
Rhesus, Cynomolgus (QC Testing)
Mouse IgG1, κ
Recombinant Human IL-4
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  6. Please refer to for technical protocols.
551774 Rev. 3
Antibody Details
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The 8D4-8 monoclonal antibody reacts with human interleukin-4 (IL-4). The immunogen used to raise the 8D4-8 hybridoma was recombinant human IL-4. The 8D4-8 antibody binds to an epitope that is different than the epitope recognized by the MP4-25D2 antibody (Cat. No. 554485).

Clone 8D4-8 displays an increased amount of non-specific binding to dead cells when compared to the clone MP4-25D2.  It is recommended to use a fixable viability dye in conjunction with this clone.

Reacts with interleukin-4 (IL-4). IL-4 is a pleiotropic cytokine produced by T cells and mast cells. It has multiple biological effects on B cells, T cells, monocytes, endothelial cells and fibroblasts. IL-4 induces the production of IgG4 and IgE in human B cells during isotype switching. Clone 8D4-8 cross-reacts with an intracellular component of SEB-stimulated peripheral blood T cells of cynomolgus and rhesus macaque monkeys. The staining patters is similar to that seen on normal human peripheral blood T cells.

551774 Rev. 3
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
551774 Rev.3
Citations & References
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Development References (2)

  1. Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
  2. Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood. 1991; 77(9):1859-1870. (Biology). View Reference
551774 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.