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PE Mouse Anti-Human IL-17RA (CD217)
PE Mouse Anti-Human IL-17RA (CD217)

Flow cytometric analysis of IL-17RA (CD217) expression on peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD217 (IL-17RA) (Cat. No. 566736; Right Plot) at 0.5 μg/test. Samples were then treated with 1× BD Pharm Lyse™ lysing solution (Cat. No. 555899) to remove erythrocytes. The contour plots showing IL-17RA (CD217) [or Ig isotype control] staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of IL-17RA (CD217) expression on peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD217 (IL-17RA) (Cat. No. 566736; Right Plot) at 0.5 μg/test. Samples were then treated with 1× BD Pharm Lyse™ lysing solution (Cat. No. 555899) to remove erythrocytes. The contour plots showing IL-17RA (CD217) [or Ig isotype control] staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
IL17RA; IL17R; interleukin-17 receptor A; IL-17 receptor A; hIL-17R; CANDF5; IMD51
Human (QC Testing)
Mouse IgG1, κ
Human IL-17RA Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2869838
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566736 Rev. 1
Antibody Details
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W23-251

The W23-251 monoclonal antibody specifically recognizes IL-17RA (CD217), which is also known as Interleukin-17 receptor A. IL-17RA (CD217) is a ~120 kDa single-pass type I transmembrane glycoprotein that is encoded by IL17RA. IL-17RA (CD217) belongs to the IL-17 receptor family which also includes IL-17 RB, IL-17RC, IL-17RD, and IL-17RE. IL-17RA (CD217) has a wide tissue distribution and is variably expressed on granulocytes, monocytes, macrophages, dendritic cells (DCs), T cells, B cells, NK cells, smooth muscle cells, endothelial cells, and epithelial cells. Interleukin-17A (IL-17A) or IL-17F homodimers, or IL-17A:IL-17F heterodimers can bind to and signal through functional IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RC subunits. This leads to the cellular release of proinflammatory cytokines including IL-1, IL-6, and TNF, antimicrobial peptides, and chemokines that attract neutrophils. IL-17E/IL-25 binds to and signals through another functional IL-17 receptor complex comprised of IL-17RA (CD217) and IL-17RB subunits. IL-17E/IL-25 induces type-2 (Th2-like) immune responses and is implicated in asthma. IL-17C binds to IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RE subunits and can stimulate the production of proinflammatory cytokines and antimicrobial peptides.

566736 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566736 Rev.1
Citations & References
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Development References (3)

  1. Gaffen SL. Structure and signalling in the IL-17 receptor family.. Nat Rev Immunol. 2009; 9(8):556-67. (Biology). View Reference
  2. Toy D, Kugler D, Wolfson M, et al. Cutting edge: interleukin 17 signals through a heteromeric receptor complex.. J Immunol. 2006; 177(1):36-9. (Biology). View Reference
  3. Yao Z, Spriggs MK, Derry JM, et al. Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine. 1997; 9(11):794-800. (Biology). View Reference
566736 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.