PE Mouse Anti-Human IDO1

BD Pharmingen™ PE Mouse Anti-Human IDO1

Name: PE Mouse Anti-Human IDO1
Brand: BD Pharmingen™
SKU: 567868

Clone V50-1886

(RUO)

Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium without (Left Plot) or with (Right Plot) lipopolysaccharide (LPS) (1 ug/ml, 20-24h, 37°C). The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm™ solution (Cat. No. 554722) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD11b (Cat. No. 562632) and PE Mouse Anti-Human IDO1 antibody (Cat. No. 567867/567868). Bivariate pseudocolor density plots showing the correlated expression of IDO1 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: IDO; IDO-1; INDO; indoleamine 2,3-dioxygenase 1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human IDO1 Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

567868 Rev. 1

Antibody Details

V50-1886

The V50-1886 monoclonal antibody specifically binds to human IDO1, or indoleamine 2, 3-dioxygenase 1, a rate-limiting enzyme in the catabolism of tryptophan. IDO1 is expressed in normal epithelial, endothelial and myeloid cells and in tumor cells. Its expression is induced by interferon gamma and increases in viral and bacterial infections. The reduction of tryptophan and increase in its metabolites that are mediated by IDO1 have critical immunosuppressive and bactericidal effects.

567868 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

567868 Rev.1

Citations & References

Development References (6)

Liu H, Shen Z, Wang Z, et al. Increased expression of IDO associates with poor postoperative clinical outcome of patients with gastric adenocarcinoma.. Sci Rep. 2016; 6:21319. (Biology). View Reference
Nakamura T1, Shima T, Saeki A, et al. Expression of indoleamine 2, 3-dioxygenase and the recruitment of Foxp3-expressing regulatory T cells in the development and progression of uterine cervical cancer.. 2007; 98(6):874-881. (Biology). View Reference
Takikawa O1, Kuroiwa T, Yamazaki F, Kido R.. Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity.. J Biol Chem. 1988; 263(4):2041-2048. (Biology). View Reference
Théate I, van Baren N, Pilotte L, et al. Extensive profiling of the expression of the indoleamine 2,3-dioxygenase 1 protein in normal and tumoral human tissues.. Cancer Immunol Res. 2015; 3(2):161-72. (Biology). View Reference
Zamorina SA, Timganova VP, Bochkova MS, Khramtsov PV, Raev MB. Effect of pregnancy-specific β1-glycoprotein on indoleamine-2,3-dioxygenase activity in human monocytes.. Dokl Biol Sci. 2016; 469(1):206-8. (Biology). View Reference
van Baren N, Van den Eynde BJ. Tryptophan-degrading enzymes in tumoral immune resistance.. Front Immunol. 2015; 6:34. (Biology). View Reference

View All (6)

567868 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.