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PE Mouse anti-Human CD325
PE Mouse anti-Human CD325

Flow cytometric analysis of N-Cadherin on H9-derived neural stem cells (NSC, left) and transformed human epithelioid carcinoma (HeLa, right). NSC derived from H9 human ES cells (WiCell, Madison, WI) and HeLa cells (ATCC CCL 2.2) were harvested without trypsinization [please note, the epitope is sensitive to trypsin] and stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Mouse Anti-Human CD325 antibody (solid line) at matching concentrations. The histograms were derived from gated events based on light scattering characteristics of the NSC or HeLa cells. Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Flow cytometric analysis of N-Cadherin on H9-derived neural stem cells (NSC, left) and transformed human epithelioid carcinoma (HeLa, right). NSC derived from H9 human ES cells (WiCell, Madison, WI) and HeLa cells (ATCC CCL 2.2) were harvested without trypsinization [please note, the epitope is sensitive to trypsin] and stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Mouse Anti-Human CD325 antibody (solid line) at matching concentrations. The histograms were derived from gated events based on light scattering characteristics of the NSC or HeLa cells. Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Product Details
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BD Pharmingen™
Cadherin-2, N-Cadherin; NCAD; CDHM; CDH2
Human (QC Testing)
Mouse IgG1, κ
Human extracellular N-Cadherin domain Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
1000
AB_10714646
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Because the extracellular domain of N-Cadherin is trypsin-sensitive, it is important to avoid using trypsin to dissociate the cells to be studied.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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8C11

The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325). Cadherins are a family of Ca2+ -dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ). Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.

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Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
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Citations & References
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Development References (3)

  1. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol. 1995; 130:66-77. (Biology). View Reference
  2. Puch S, Armeanu S, Kibler C, et al. N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. J Cell Sci. 2001; 114(8):1567-1577. (Clone-specific: Flow cytometry). View Reference
  3. Wein F, Pietsch L, Saffrich R, et al. N-Cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells. Stem Cell Res. 2010; 4(2):129-139. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.