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PE Mouse Anti-Human CD218a (IL-18Rα)
PE Mouse Anti-Human CD218a (IL-18Rα)

Multicolor flow cytometric analysis of CD218a (IL-18Rα) expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD8 (Cat. No. 555366/557085/561947/561948; Left Panel) and APC Mouse Anti-Human CD56 (Cat. No. 555518; Right Panel) antibodies and either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; Left Plots) or PE Mouse Anti-Human CD218a (IL-18Rα) antibody (Cat. No. 564675; Right Plots). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).  The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 or CD56 versus CD218a (IL-18Rα) [or Ig Isotype control staining] were derived from gated events with the forward and side-light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Multicolor flow cytometric analysis of CD218a (IL-18Rα) expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD8 (Cat. No. 555366/557085/561947/561948; Left Panel) and APC Mouse Anti-Human CD56 (Cat. No. 555518; Right Panel) antibodies and either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; Left Plots) or PE Mouse Anti-Human CD218a (IL-18Rα) antibody (Cat. No. 564675; Right Plots). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).  The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 or CD56 versus CD218a (IL-18Rα) [or Ig Isotype control staining] were derived from gated events with the forward and side-light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
IL-1Rrp; IL1RRP; IL18RA; IL-18Rα; IL-18R1; IL-18R-1; IL18R1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human NK Cell Line
Flow cytometry (Routinely Tested)
5 µl
VIII 80438
AB_2738885
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
564675 Rev. 1
Antibody Details
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H44

The H44 monoclonal antibody specifically binds to CD218a, which is also known as Interleukin-18 receptor 1 (IL-18R1), IL-18 Receptor alpha (IL-18Rα) and IL1 Receptor-related protein (IL-1Rrp). CD218a is a type I transmembrane glycoprotein that associates with CD218b (IL-18Rβ) subunit to form the functional IL-18 Receptor Complex. Both subunits belong to the IL-1R family. The IL-18Rα chain is responsible for extracellular IL-18 binding whereas the IL-18Rβ subunit is involved in intracellular signaling through the NFκB pathway. CD218a is expressed on a variety of cells including T cells, NK cells, macrophages, granulocytes, keratinocytes, endothelial cells, and smooth muscle cells. CD218a expression is induced weakly in T cells by IL-12 treatment and moderately by phytohemagglutinin (PHA) stimulation. Costimulation with both IL-12 and PHA induces optimal CD218a expression on CD4+ T cells, CD8+ T cells, and CD56+ NK cells. The IL-18/IL-18R Complex plays important roles in adaptive immunity as well as innate immune responses and autoimmunity. H44 reportedly neutralized the biological activity of recombinant human IL-18 protein.

564675 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564675 Rev.1
Citations & References
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Development References (6)

  1. Kitasato Y, Hoshino T, Okamoto M, et al. Enhanced expression of interleukin-18 and its receptor in idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. 2004; 31(6):619-625. (Immunogen: Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Inhibition, Neutralization, Radioimmunoassay). View Reference
  2. Kunikata T, Torigoe K, Ushio S, et al. Constitutive and induced IL-18 receptor expression by various peripheral blood cell subsets as determined by anti-hIL-18R monoclonal antibody. Cell Immunol. 1998; 189(2):135-143. (Biology). View Reference
  3. Nakamura S, Otani T, Okura R, et al. Expression and responsiveness of human interleukin-18 receptor (IL-18R) onhematopoietic cell lines. Leukemia. 2000 ; 14(6):1052-1059. (Biology). View Reference
  4. Parnet P, Garka KE, Bonnert TP, Dower SK, Sims JE. IL-1Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R AcP. J Biol Chem. 1996; 271(8):3967-3970. (Biology). View Reference
  5. Torigoe K, Ushio S, Okura T, et al. Purification and characterization of the human interleukin-18 receptor. J Biol Chem. 1997; 272(41):25737-25742. (Biology). View Reference
  6. Vermot-Desroches C, Subiger O, Guenot F, Sergent E, Bonnin B, Wijdenes J. Monoclonal antibodies specific for the IL-18 receptor. Cell Immunol. 2005; 236(1-2):101-104. (Clone-specific: ELISA, Flow cytometry). View Reference
View All (6) View Less
564675 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.