The 32/SHIP-1 monoclonal antibody specifically recognizes the cytosolic lipid phosphatase, SH2 domain-containing inositol phosphatase 1 (SHIP-1), which is also known as Inpp5d. SHIP-1 is expressed in cells of the hematopoietic lineage. Cells can phosphorylate and activate SHIP-1 in response to a variety of cytokines or triggering through B or T cell antigen receptors. Activated SHIP-1 hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. This ultimately blocks the recruitment of PH-domain containing effector proteins, such as Btk and Akt, and can prevent cellular activation.
This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg, 610/20-nm filter).