The LB-2 monoclonal antibody specifically binds to CD54 which is also known as Intercellular adhesion molecule-1 (ICAM-1). CD54 is an 85-110 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin supergene family. A soluble form of CD54 can also be found in biological fluids. CD54 is expressed on endothelial cells and both resting (weak) and activated (moderate) T and B lymphocytes and monocytes. CD54 is expressed on human myeloma cells, Burkitt's lymphoma cells, erythroleukemia cells, and B-lymphoblastoid cells. CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) and can act as a receptor for Rhinoviruses. The CD54 adhesion molecule plays roles in inflammatory and immune responses and neoplasia.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.