The 1A5 monoclonal antibody specifically recognizes Sialic acid binding Ig-like lectin 5 (Siglec-5) which is also known as CD170, CD33 antigen-like 2 (CD33L2) and Obesity binding protein-2 (OBBP2). CD170 is a type I transmembrane glycoprotein that forms a ~140 kDa dimer on the cell surface. CD170 is encoded by SIGLEC5 which belongs to the SIGLEC (sialic acid binding Ig-like lectin) family within the immunoglobulin superfamily. The extracellular region of CD170 consists of an N-terminal V-set Ig-like domain, which contains the sialic acid binding site, followed by 3 C2-set Ig like domains, a transmembrane region and a cytoplasmic tail containing 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) which regulate cellular signaling. CD170 bind to alpha-2,3- and alpha-2,6-linked sialic acid glycoproteins and glycolipids and to glycophorin A. It is highly expressed on neutrophils and expressed at lower levels on monocytes, macrophages, dendritic cells, and some lymphocytes. CD170 is an adhesion molecule that may function in regulating cellular activation and in sialic-acid dependent binding to cells.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.