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BV421 Rat Anti-Mouse CD317 (BST2)
BV421 Rat Anti-Mouse CD317 (BST2)
Two-color flow cytometric analysis of CD317 (BST2) expression on mouse splenocytes. SJL mouse spleen cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either BD Horizon™ BV421 Rat IgG2b, κ Isotype Control (Cat. No. 562603; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD317 (BST2) antibody (Cat. No. 566431; Right Plot) at 0.06 μg/test. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 versus CD317 (BST2) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD317 (BST2) expression on mouse splenocytes. SJL mouse spleen cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either BD Horizon™ BV421 Rat IgG2b, κ Isotype Control (Cat. No. 562603; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD317 (BST2) antibody (Cat. No. 566431; Right Plot) at 0.06 μg/test. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 versus CD317 (BST2) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
Bst2; BST-2; PDCA-1; PDCA1; Tetherin; DAMP-1; GREG
Mouse (QC Testing)
Rat IgG2b, κ
Mouse bone marrow-derived type I IFN-producing cella (IPC)
Flow cytometry (Routinely Tested)
0.2 mg/ml
69550
AB_2739728
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566431 Rev. 1
Antibody Details
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927

The 927 monoclonal antibody specifically recognizes CD317 which is also known as Bone marrow stromal antigen 2 (BST2) or Plasmacytoid Dendritic Cell Ag-1 (PDCA-1). CD317 (BST2) is a type II transmembrane glycoprotein that is encoded by Bst2. It is highly expressed on naïve plasmacytoid dendritic cells (pDC) which comprise very small populations within lymphoid and nonlymphoid tissues. pDC are characteristically early responders to viruses through Toll-like receptors (TLR), TLR7 and TLR9. Activated pDC can produce large amounts of proinflammatory cytokines including type I interferons, Interferon-alpha (IFN-α) and Interferon-beta (IFN-β), and are also known as Type I IFN producing cells (IPC). CD317 (BST2) expression can be upregulated on a variety of other cell types following stimulation with type I IFNs and IFN gamma including T cells, B cells, plasma cells, NK cells, CD8+ and CD8- dendritic cells, and cell lines. CD317 (BST2) may play a role in sorting proteins, eg, cytokines, from the Golgi apparatus and the plasma membrane.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566431 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566431 Rev.1
Citations & References
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Development References (2)

  1. Blasius AL, Giurisato E, Cella M, Schreiber RD, Shaw AS, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation.. J Immunol. 2006; 177(5):3260-5. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunofluorescence). View Reference
  2. Ohtomo T, Sugamata Y, Ozaki Y, et al. Molecular cloning and characterization of a surface antigen preferentially overexpressed on multiple myeloma cells. Biochem Biophys Res Commun. 1999; 258(3):583-591. (Biology). View Reference
566431 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.