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BV421 Mouse Anti-Rat Ig, κ Light Chain
BV421 Mouse Anti-Rat Ig, κ Light Chain
Multiparameter flow cytometric analysis using BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) and BD OptiBuild™ BV421 Mouse Anti-Rat Ig, κ light chain (Cat. No. 743808; Right Plot) on live Rat splenic leukocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
BV421 Mouse Anti-Rat Ig, κ Light Chain
Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat Ig, κ light chain (Cat. No. 743808, right panel) on live rat splenocytes, with Isotype Control (left panel). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
Multiparameter flow cytometric analysis using BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) and BD OptiBuild™ BV421 Mouse Anti-Rat Ig, κ light chain (Cat. No. 743808; Right Plot) on live Rat splenic leukocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat Ig, κ light chain (Cat. No. 743808, right panel) on live rat splenocytes, with Isotype Control (left panel). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
Product Details
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BD OptiBuild™
Igkc; Ig κ light chain; Ig kappa light chain
Rat (Tested in Development)
Mouse IgG1
Pooled rat Ig
Flow cytometry (Qualified)
0.2 mg/ml
AB_2741769
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. For U.S. patents that may apply, see bd.com/patents.
743808 Rev. 2
Antibody Details
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MRK-1

The MRK-1 monoclonal antibody reacts specifically with rat Igs bearing κ light chains. It does not react with rat immunoglobulin λ light chains or heavy chains. The MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

743808 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
743808 Rev.2
Citations & References
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Development References (3)

  1. Bliss SK, Butcher BA, Denkers EY. Rapid recruitment of neutrophils containing prestored IL-12 during microbial infection.. J Immunol. 2000; 165(8):4515-21. (Clone-specific: Flow cytometry). View Reference
  2. Goetzl EJ, Dembrow D, Van Brocklyn JR, Gráler M, Huang MC. An IgM-kappa rat monoclonal antibody specific for the type 1 sphingosine 1-phosphate G protein-coupled receptor with antagonist and agonist activities.. Immunol Lett. 2004; 93(1):63-9. (Clone-specific: Radioimmunoassay). View Reference
  3. Igarashi H, Kuwata N, Kiyota K, et al. Localization of recombination activating gene 1/green fluorescent protein (RAG1/GFP) expression in secondary lymphoid organs after immunization with T-dependent antigens in rag1/gfp knockin mice.. Blood. 2001; 97(9):2680-7. (Clone-specific). View Reference
743808 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.