Skip to main content Skip to navigation
BV421 Mouse Anti-Human STING
BV421 Mouse Anti-Human STING

Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with PE Mouse Anti-Human CD20 antibody (Cat. No. 561174) and either BD Horizon™ BV421 IgG1, κ Isotype Control (Cat. No. 562438; Top Panels) or BD Horizon BV421 Mouse Anti-Human STING antibody (Cat. No. 564966; Bottom Panels).

     Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes.

      Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

     Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with PE Mouse Anti-Human CD20 antibody (Cat. No. 561174) and either BD Horizon™ BV421 IgG1, κ Isotype Control (Cat. No. 562438; Top Panels) or BD Horizon BV421 Mouse Anti-Human STING antibody (Cat. No. 564966; Bottom Panels).

     Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes.

      Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

     Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Product Details
Down Arrow Up Arrow


BD Horizon™
hSTING; MITA; hMITA; ERIS; MPYS; NET23; TM173; TMEM173
Human (QC Testing)
Mouse IgG1, κ
Human STING Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2739027
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564966 Rev. 1
Antibody Details
Down Arrow Up Arrow
T3-680

The T3-680 monoclonal antibody specifically recognizes human STING (Stimulator of interferon genes protein) which is also known as Endoplasmic reticulum interferon stimulator (ERIS) and Mediator of IRF3 activation (MITA). STING is encoded by TMEM173 (Transmembrane protein 173). STING is a multi-pass transmembrane adaptor protein which is widely expressed in the endoplasmic reticulum of cells. STING acts as a sensor of cytosolic double-stranded DNA derived from bacteria and viruses and promotes the production of type I interferons, IFN-α and IFN-β. Following activation, STING translocates with TBK1 kinase to perinuclear endosomes. The TBK1 kinase phosphorylates and activates interferon regulatory factors, such as IRF3, and NF-κB, which leads to the induction of type I interferon and other immune response genes that play roles in innate and adaptive immunity.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

564966 Rev. 1
Format Details
Down Arrow Up Arrow
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
564966 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Ablasser A, Goldeck M, Cavlar T, et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature. 2013; 498(7454):380-384. (Biology). View Reference
  2. Ishikawa H, Barber GN. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature. 2008; 455(7213):674-678. (Biology). View Reference
  3. Sun L, Wu J, Du F, Chen X, Chen ZJ. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 2012; 339(6121):786-791. (Biology). View Reference
  4. Unterholzner L, Keating SE, Baran M, et al. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 2010; 11(11):997-1004. (Biology). View Reference
  5. Zhang Z, Yuan B, Bao M, Lu N, Kim T, Liu YJ. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol. 2012; 12(10):959-965. (Biology). View Reference
View All (5) View Less
564966 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.