BV421 Mouse Anti-Human IgE
Two-color flow cytometric analysis of IgE expression on human peripheral blood leucocytes. Whole blood cells were stained with BD Horizon™ PE-CF594 Mouse Anti-Human CD193 antibody (Cat. No. 562571) and either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; Left Plot) or BD Horizon BV421 Mouse Anti-Human IgE antibody (Cat. No. 566324/566325; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric dot plot showing the correlated expression of CD193 versus IgE (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Horizon™ BV421 Mouse Anti-Human IgE
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