The 186107 monoclonal antibody specifically recognizes the Retinoic acid early inducible 1 (Rae-1) family of glycoproteins which is also known as retinoic acid early transcript 1. This family includes Rae-1α, β, δ, ε and γ, which are encoded by Raet1a, Raet1b, Raet1d, Raet1e, and Raet1c, respectively. Although expressed by some cells during mouse embryonic development, expression of these highly homologous membrane proteins is either low or absent on cells within adult tissues. These glycophosphatidylinositol (GPI)-linked proteins are structurally related to MHC class I molecules and contain alpha 1 and 2 domains. The Rae-1 family of glycoproteins serve as ligands for CD314 (NKG2D) which functions as an activating receptor expressed on some cytotoxic T cells and NK cells. Stress-induced Rae-1 proteins are expressed on tumor cells and might play a role in tumor rejection.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.