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BUV496 Mouse Anti-Human HLA-C
Product Details
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BD OptiBuild™
HLAC; HLC-C; HLA-JY3; MHC class I antigen C; PSORS1; HLA-E
Human (Tested in Development)
Mouse IgG2b, κ
Purified Human Tamarin MHC Class I Molecules
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV496 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
750377 Rev. 3
Antibody Details
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The DT-9 monoclonal antibody specifically recognizes Human Leukocyte Antigen (HLA-C), a polymorphic major histocompatibility complex (MHC) class I antigen. HLA-C is a heterodimer comprised of the HLA-C alpha chain, a ~45 kDa type I transmembrane glycoprotein, and a ~12 kDa Beta-2 (β2)-microglobulin light chain. HLA-C is expressed on nearly all cells, and plays a role in the antigen-specific, MHC-restricted presentation of small peptides to CD8+ T cells in the generation of immunity or tolerance. HLA-C may also bind to regulatory MHC class I antigen-selective receptors expressed by CD8+ T cells and natural killer (NK) cells.  The DT-9 antibody also recognizes some rare HLA-A and HLA-B allotypes and the nonclassical HLA-E molecule.

The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.

750377 Rev. 3
Format Details
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The BD Horizon Brilliant™ Ultraviolet 496 (BUV496) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 496-nm. BUV496, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 500-nm (e.g., 515/30-nm bandpass filter). The acceptor dye can be excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Ultraviolet 355 nm
350 nm
496 nm
750377 Rev.3
Citations & References
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Development References (6)

  1. Apps R, Qi Y, Carlson JM, et al. Influence of HLA-C expression level on HIV control.. Science. 2013; 340(6128):87-91. (Clone-specific: Flow cytometry). View Reference
  2. Braud VM, Allan DS, O'Callaghan CA, et al. HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C.. Nature. 1998; 391(6669):795-9. (Clone-specific: Flow cytometry). View Reference
  3. Braud VM, Allan DS, Wilson D, McMichael AJ. TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide.. Curr Biol. 1998; 8(1):1-10. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
  4. Corrah TW, Goonetilleke N, Kopycinski J, et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression.. J Virol. 2011; 85(7):3367-74. (Clone-specific: Flow cytometry). View Reference
  5. Thomas R, Apps R, Qi Y, et al. HLA-C cell surface expression and control of HIV/AIDS correlate with a variant upstream of HLA-C.. Nat Genet. 2009; 41(12):1290-4. (Clone-specific: Flow cytometry). View Reference
  6. Zipeto D, Beretta A. HLA-C and HIV-1: friends or foes?. Retrovirology. 2012; 9:39. (Biology). View Reference
View All (6) View Less
750377 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.