Skip to main content Skip to navigation
BUV395 Mouse Anti-Mouse CD244.2
Product Details
Down Arrow Up Arrow

BD OptiBuild™
CD242.2; 2B4 B6 Alloantigen; Ly90; NAIL; Nmrk; SLAMF4
Mouse (Tested in Development)
Mouse 129, also known as 129/J or 129/SvJ IgG2b, κ
rIL-2-propagated NK1.1+ cells from C57BL/6 mice
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
740226 Rev. 1
Antibody Details
Down Arrow Up Arrow

CD244 is a type I transmembrane glycoprotein that is also known as Natural killer cell receptor 2B4 (NKR2B4), or Signaling lymphocytic activation molecule 4 (SLAMF4). CD244 is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2-activated NK (LAK) cells, committed progenitors of NK cells, and a subset of T lymphocytes which mediate non-MHC-restricted cytoxicity, including dendritic epidermal T cells. The 2B4 monoclonal antibody specifically binds to CD244.2, the 2B4 alloantigen which is expressed on C57BL/6 and C58/J mice, but not in most strains tested (A/J, AKR, BALB/c, CBA/J, CBA/N, C3H/He, C57BR, DBA/1, DBA/2, NZB, SJL/J, 129). At least two isoforms of CD244.2 protein, products of alternative splicing of hnRNA, are expressed on IL-2-activated C57BL/6 NK cells. They differ only in their cytoplasmic domains; with 2B4L (150-aa cytoplasmic tail) having inhibitory activity and 2B4S (93-aa tail) being stimulatory. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. 2B4 antibody activates lytic and secretory functions of IL-2-cultured NK cells and CD244.2+ T cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

740226 Rev. 1
Format Details
Down Arrow Up Arrow
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Ultraviolet 355 nm
348 nm
395 nm
740226 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (13)

  1. Brown MH, Boles K, van der Merwe PA, Kumar V, Mathew PA, Barclay AN. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J Exp Med. 1998; 188(11):2083-2090. (Biology). View Reference
  2. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
  3. Garni-Wagner BA, Purohit A, Mathew PA, Bennett M, Kumar V. A novel function-associated molecule related to non-MHC-restricted cytotoxicity mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(1):60-70. (Biology). View Reference
  4. Kubota K, Katoh H, Muguruma K, Koyama K. Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen. Immunology. 1999; 96(3):491-497. (Biology). View Reference
  5. Kumaresan PR, Stepp SE, Bennett M, Kumar V, Mathew PA. Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. Immunogenetics. 2000; 51(4-5):306-313. (Biology). View Reference
  6. Latchman Y, McKay PF, Reiser H. Identification of the 2B4 molecule as a counter-receptor for CD48. J Immunol. 1998; 161(11):5809-5812. (Biology). View Reference
  7. Mathew PA, Garni-Wagner BA, Land K, et al. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(10):5328-5337. (Biology). View Reference
  8. Rosmaraki EE, Douagi I, Roth C, Colucci F, Cumano A, Di Santo JP. Identification of committed NK cell progenitors in adult murine bone marrow. Eur J Immunol. 2001; 31(6):1900-1909. (Biology). View Reference
  9. Schatzle JD, Sheu S, Stepp SE, Mathew PA, Bennett M, Kumar V. Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4. Proc Natl Acad Sci U S A. 1999; 96(7):3870-3875. (Biology). View Reference
  10. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. 2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors. J Invest Dermatol. 1995; 105(4):592-596. (Biology). View Reference
  11. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. Activation of murine epidermal gamma delta T cells through surface 2B4. Eur J Immunol. 1995; 25(4):1117-1120. (Biology). View Reference
  12. Sentman CL, Hackett J Jr, Moore TA, Tutt MM, Bennett M, Kumar V. Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen. Hybridoma. 1989; 8(6):605-614. (Immunogen). View Reference
  13. Stepp SE, Schatzle JD, Bennett M, Kumar V, Mathew PA. Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains. Eur J Immunol. 1999; 29(8):2392-2399. (Biology). View Reference
View All (13) View Less
740226 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.