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Biotin Mouse Anti-Rat IgG2b
Product Details
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BD Pharmingen™
Ig gamma-2B; Immunoglobulin G2b; Cγ2b
Rat (QC Testing)
Mouse IgG2b, κ
Pooled rat Ig
ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

Flow cytometry: Biotin G15-337 mAb may be used as a secondary reagent in immunofluorescent staining. G15-337 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC-conjugated G15-337 mAb (Cat. No. 553884).

ELISA: For the sandwich rat IgG2b ELISA, purified G15-337 mAb (Cat. No. 553882) is optimal for capture with biotinylated anti-rat IgG2b RG7/11.1 mAb (Cat. No. 553898) for detection.

IHC: For immunohistochemical staining of acetone-fixed frozen sections, we recommend the use of biotinylated G15-337 mAb in our special formulation for immunohistochemistry, Cat. No. 550327.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  4. Please refer to for technical protocols.
553883 Rev. 13
Antibody Details
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The G15-337 monoclonal antibody specifically recognizes rat IgG2b. It does not react with other Ig isotypes. A suspension of pooled rat Ig was used as the source of immunogen.

553883 Rev. 13
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553883 Rev.13
Citations & References
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Development References (2)

  1. Pérez-Berezo T, Franch A, Castellote C, Castell M, Pérez-Cano FJ. Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake.. J Nutr Biochem. 2012; 23(7):838-44. (Biology). View Reference
  2. Stenstad H, Svensson M, Cucak H, Kotarsky K, Agace WW. Differential homing mechanisms regulate regionalized effector CD8alphabeta+ T cell accumulation within the small intestine.. Proc Natl Acad Sci USA. 2007; 104(24):10122-7. (Clone-specific). View Reference
553883 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.