Flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD57 (Cat. No. 560845; solid line histogram) or with an APC Mouse IgM, κ Isotype Control (Cat. No. 550883; dashed line histogram). The erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
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Flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD57 (Cat. No. 560845; solid line histogram) or with an APC Mouse IgM, κ Isotype Control (Cat. No. 550883; dashed line histogram). The erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
Product Details
BD Pharmingen™
B3GAT1; Beta-1,3-glucuronyltransferase 1; HNK1; LEU7; NK-1; GLCATP; GLCUATP
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
5 µl
AB_10563760
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
APC Mouse IgM, κ Isotype Control RUO
Size
0.1 mg
Cat No.
550883
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560845 Rev. 2
Antibody Details
NK-1
The NK-1 monoclonal antibody specifically reacts with a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein expressed on 7-35% of normal peripheral blood lymphocytes including a subset of natural killer cells, a subset of CD8-positive peripheral blood T cells, and on some neural tissues. CD57 is not expressed on granulocytes, platelets, red blood cells or thymocytes. The function of CD57 is still unclear, however, its expression on T-cell subets occurs in late immune responses.
560845 Rev. 2
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
560845 Rev.2
Citations & References
Development References (5)
-
Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981; 127(3):1024-1029. (Biology). View Reference
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Abo T, Cooper MD, Balch CM. Characterization of HNK-1+ (Leu-7) human lymphocytes. I. Two distinct phenotypes of human NK cells with different cytotoxic capability. J Immunol. 1982; 129(4):1752-1757. (Biology). View Reference
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McGarry RC, Helfand SL, Quarles RH, Roder JC. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature. 1983; 306(5941):376-378. (Biology). View Reference
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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d'Angeac AD, Monier S, Pilling D, Travaglio-Encinoza A, Reme T, Salmon M. CD57+ T lymphocytes are derived from CD57- precursors by differentiation occurring in late immune responses. Eur J Immunol. 1994; 24(7):1503-1511. (Biology). View Reference
560845 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
