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Alexa Fluor® 700 Rat Anti-Mouse Vα2 TCR
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Alexa Fluor® 700 Rat Anti-Mouse Vα2 TCR

Flow cytometric analysis of Vα2 TcR expression on mouse lymph node cells.  Lymph node cells from BALB/c mice were stained with APC-Cy™7 Rat anti-Mouse CD4 (Cat. No. 552051) and APC-Cy™7 Rat anti-Mouse CD8a (Cat. No. 557654) in addition to either an Alexa Fluor® 700 Rat IgG2a, λ isotype control (Cat. No. 557963, Left Panel) or with the Alexa Fluor® 700 Rat anti-Mouse Vα2 TCR antibody (Cat. No. 561239, Right Panel).  Two-color flow cytometric dot plots showing the correlated expression of Vα2 TCR versus CD4 and CD8a were derived from gated events with the forward and side light-scattering characteristics of viable lymph node cells.  Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.

Flow cytometric analysis of Vα2 TcR expression on mouse lymph node cells.  Lymph node cells from BALB/c mice were stained with APC-Cy™7 Rat anti-Mouse CD4 (Cat. No. 552051) and APC-Cy™7 Rat anti-Mouse CD8a (Cat. No. 557654) in addition to either an Alexa Fluor® 700 Rat IgG2a, λ isotype control (Cat. No. 557963, Left Panel) or with the Alexa Fluor® 700 Rat anti-Mouse Vα2 TCR antibody (Cat. No. 561239, Right Panel).  Two-color flow cytometric dot plots showing the correlated expression of Vα2 TCR versus CD4 and CD8a were derived from gated events with the forward and side light-scattering characteristics of viable lymph node cells.  Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
Flow cytometry (Routinely Tested)
0.2 mg/ml
111660
AB_10611715
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561239 Rev. 1
Antibody Details
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B20.1

The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes.

561239 Rev. 1
Format Details
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Alexa Fluor™ 700
Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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Alexa Fluor™ 700
Red 627-640 nm
697 nm
719 nm
561239 Rev.1
Citations & References
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Development References (3)

  1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Biology). View Reference
  2. Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). View Reference
  3. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
561239 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.