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Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen
Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen

Flow cytometric analysis for T- and B- Cell Activation Antigen in activated mouse spleen cells. Concanavalin A-stimulated (3 days) BALB/c mouse spleen cells were incubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142) and were then stained either with an Alexa Fluor® 647 Rat IgM, κ Isotype Control (Cat No. 560892; dashed line histogram) or with the Alexa Fluor® 647 Rat Anti-Mouse T- and B- Cell Activation Antigen (Cat No. 561529; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphoblasts. Flow cytometry was performed on a BD™ LSR II.

Flow cytometric analysis for T- and B- Cell Activation Antigen in activated mouse spleen cells. Concanavalin A-stimulated (3 days) BALB/c mouse spleen cells were incubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142) and were then stained either with an Alexa Fluor® 647 Rat IgM, κ Isotype Control (Cat No. 560892; dashed line histogram) or with the Alexa Fluor® 647 Rat Anti-Mouse T- and B- Cell Activation Antigen (Cat No. 561529; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphoblasts. Flow cytometry was performed on a BD™ LSR II.

Product Details
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BD Pharmingen™
GL7; GL-7; GL7 Antigen
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgM, κ
In vitro-activated T-cell-depleted CBA/Ca mouse splenocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10716056
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561529 Rev. 2
Antibody Details
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GL7

The GL7 antibody specifically recognizes the T- and B- Cell Activation Antigen which is also known as, the GL7 antigen. The GL7 antigen is a 35-kDa cell-surface protein that is expressed on T and B lymphocytes activated in vitro, on bone marrow Pre-B-II cells, germinal-center B cells, and the subpopulation of thymocytes that coexpress high CD3e levels.  The GL7 antibody recognizes an epitope containing nonsulfated α2-6-sialyl-LacNAc. There is strain variability with respect to GL7 antigen distribution on thymocytes and Con A-activated spleen cells. GL7 antigen expression is found to be higher on BALB/c mouse leucocytes than on C57BL/6 mouse counterparts. The GL7 antibody reportedly may crossreact with epitopes on molecules expressed by certain rat and human leucocyte subsets.

561529 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
561529 Rev.2
Citations & References
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Development References (5)

  1. Han S, Dillon SR, Zheng B, Shimoda M, Schlissel MS, Kelsoe G. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. 1997; 278(5336):301-305. (Biology). View Reference
  2. Han S, Zheng B, Schatz DG, Spanopoulou E, Kelsoe G. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science. 1996; 274(5295):2094-2097. (Biology). View Reference
  3. Han S, Zheng B, Takahashi Y, Kelsoe G. Distinctive characteristics of germinal center B cells. Semin Immunol. 1997; 9(4):255-260. (Biology). View Reference
  4. Hathcock KS, Pucillo CE, Laszlo G, Lai L, Hodes RJ. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J Immunol. 1995; 155(10):4575-4581. (Biology). View Reference
  5. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Immunogen). View Reference
View All (5) View Less
561529 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.