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Alexa Fluor® 647 Rat anti-Mouse CD371 (Clec12A)
Alexa Fluor® 647 Rat anti-Mouse CD371 (Clec12A)

Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dashed line histogram) or Alexa Fluor® 647 Rat Anti-Mouse Clec12A (Cat. No. 564794; solid line histogram). Overlapping histograms showing Clec12A expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scattering characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed on a BD™ LSR II.

Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dashed line histogram) or Alexa Fluor® 647 Rat Anti-Mouse Clec12A (Cat. No. 564794; solid line histogram). Overlapping histograms showing Clec12A expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scattering characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed on a BD™ LSR II.

Product Details
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BD Pharmingen™
CD371; CL12A; CLL-1; KLRL1; MICL; Micl; D230024O04
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse Clec12A Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738954
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564794 Rev. 4
Antibody Details
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5D3/CD371

The 5D3/CD371 (aka, 1/06-5D3) monoclonal antibody specifically binds to mouse CD371 which is encoded by Clec12A (C-type lectin domain family 12 member A). CD371 is also known as Myeloid inhibitory C-type lectin-like receptor (MICL), C-type lectin-like molecule 1 (CLL-1) or Dendritic cell-associated lectin 2 (DCAL-2). CD371 is a ~30 kDa type II transmembrane glycoprotein that possesses one extracellular C-type lectin domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). CD371 is expressed on plasmacytoid dendritic cells, subsets of myeloid dendritic cells, monocytes, macrophages and B cells. It reportedly serves as a negative regulator of granulocyte and monocyte function.

564794 Rev. 4
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564794 Rev.4
Citations & References
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Development References (4)

  1. Lahoud MH, Proietto AI, Ahmet F, et al. The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses.. J Immunol. 2009; 182(12):7587-94. (Immunogen: Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, In vivo exacerbation, Stimulation). View Reference
  2. Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). View Reference
  3. Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). View Reference
  4. van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). View Reference
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564794 Rev. 4

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.