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Alexa Fluor™ 647 Mouse Anti-Islet-1
Alexa Fluor™ 647 Mouse Anti-Islet-1
Flow cytometric analysis of Islet-1 in Mouse pancreatic tumor (Insulinoma) cells.  Cells from the Mouse Beta-TC-6 (Insulinoma, ATCC© CRL-3605™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either Alexa Flour™ 647 Mouse IgG1, κ isotype control (Cat. No. 570232; dashed line histogram) or Alexa Flour™ 647 Mouse Anti-Islet-1 antibody (Cat. No. 570357/570427; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing Islet-1 expression (or Ig Isotype control staining) was derived was from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSCanto™ II Flow Cytometry System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Islet-1 in Mouse pancreatic tumor (Insulinoma) cells.  Cells from the Mouse Beta-TC-6 (Insulinoma, ATCC© CRL-3605™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either Alexa Flour™ 647 Mouse IgG1, κ isotype control (Cat. No. 570232; dashed line histogram) or Alexa Flour™ 647 Mouse Anti-Islet-1 antibody (Cat. No. 570357/570427; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing Islet-1 expression (or Ig Isotype control staining) was derived was from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSCanto™ II Flow Cytometry System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
ISL-1, ISL1, Islet-2, ISL-2, ISL2
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Human Islet-1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
3670, 16392
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  11. For U.S. patents that may apply, see bd.com/patents.
570427 Rev. 1
Antibody Details
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Q11-465

Islet-1 is a LIM-homeodomain transcription factor important for motor neuron differentiation and the formation of islet cells in the pancreas. Various heart cell types, such as cardiac muscle, the conduction system and endothelial cells in multiple heart tissue compartments during cardiogenesis, have been found to originate from Islet-1-positive cardiac precursor cells. Moreover, Islet-1-positive cells from differentiated human embryonic stem cell lines were found to be capable of self-renewal and expansion and could differentiate into the three major cell types of the heart.

Western blot analyses of lysates from transfected cells demonstrate that the Q11-465 monoclonal antibody reacts with human Islet-1 (ISL-1, ISL1) and Islet-2 (ISL-2, ISL2), similar to the 4D5 clone (Tsuchida et al, 1994). Cross-reactivity with mouse Islet is also observed.

570427 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
570427 Rev.1
Citations & References
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View product citations for antibody "570427" on CiteAb

Development References (7)

  1. Bu L, Jiang X, Martin-Puig S, et al. Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature. 2009; 460(7251):113-117. (Biology). View Reference
  2. Ebert AD, Yu J, Rose FF Jr, et al. Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature. 2009; 457(7227):277-280. (Biology). View Reference
  3. Emre N, Vidal JG, Elia J, et al. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS ONE. 2010; 5(8):e12148. (Methodology: Cell differentiation). View Reference
  4. Laugwitz KL, Moretti A, Caron L, Nakano A, Chien KR. Islet1 cardiovascular progenitors: a single source for heart lineages. Development. 2008; 135(2):193-205. (Biology). View Reference
  5. Osumi N, Hirota A, Ohuchi H, et al. Pax-6 is involved in the specification of hindbrain motor neuron subtype. Development. 1997; 124(15):2961-2972. (Biology). View Reference
  6. Tsuchida T, Ensini M, Morton SB, et al. Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. Cell. 1994; 79(6):957-970. (Biology). View Reference
  7. Xu C, Police S, Hassanipour M, et al. Efficient generation and cryopreservation of cardiomyocytes derived from human embryonic stem cells. 2011; 6(1):53-66. (Biology). View Reference
View All (7) View Less
570427 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.