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      Alexa Fluor™ 647 Mouse Anti-Human TCR Vβ13.2
      Alexa Fluor™ 647 Mouse Anti-Human TCR Vβ13.2
      Two-color flow cytometric analysis of TCR Vβ13.2 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BUV395 Mouse Anti-Human CD3 antibody (Cat. No. 563546) and with either Alexa Fluor 647™ Mouse IgG1, κ Isotype Control (Cat. No. 565571; Left Plot) or Alexa Fluor™ 647 Mouse Anti-Human TCR Vβ13.2 antibody (Cat. No. 568086/568087; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TCR Vβ13.2 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      Alexa Fluor™ 647 Mouse Anti-Human TCR Vβ13.2
      Two-color flow cytometric analysis of TCR Vβ13.2 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BUV395 Mouse Anti-Human CD3 antibody (Cat. No. 563546) and with either Alexa Fluor 647™ Mouse IgG1, κ Isotype Control (Cat. No. 565571; Left Plot) or Alexa Fluor™ 647 Mouse Anti-Human TCR Vβ13.2 antibody (Cat. No. 568086/568087; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TCR Vβ13.2 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      Product Details
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      BD Pharmingen™
      TCR V beta 13.2; TCR Vb13.2; TCRBV13S2; TCRBV6S2; TRBV6-2; TRBV62
      Human (QC Testing)
      Mouse SWR IgG1, κ
      Human TCR Vβ13.2 Element Transfected Cell Line
      Flow cytometry (Routinely Tested)
      5 µl/test
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      9. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      10. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
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      568086 Rev. 1
      Antibody Details
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      H132

      The H132 monoclonal antibody specifically recognizes the variable beta 13.2 region of the β subunit of the human αβ T cell receptor for antigen (TCR Vβ13.2). TCR Vβ13.2 is expressed on subsets of TCR αβ positive thymocytes and peripheral CD4+ and CD8+ T cells. The H132 antibody is useful for multiparameter analyses designed to study the nature of TCR Vβ13.2-positive cells including normal T cells as well as T cell clones, hybridomas, or tumors. It is also useful for analyzing TCR Vβ repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, autoimmunity, responses to superantigens, tumors, and infectious diseases including HIV infection. The H132 antibody can reportedly stimulate the proliferation of TCR Vβ13.2-positive T cells. The H132 antibody recognizes human TCR Vβ13.2 subfamily members but does not react with other human TCR Vβ13 family members including TCR Vβ13.1, Vβ13.3, Vβ13.5 and Vβ13.6 subfamily members.

      568086 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      568086 Rev.1
      Citations & References
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      Development References (6)

      1. Choi YW, Kotzin B, Lafferty J, et al. A method for production of antibodies to human T-cell receptor beta-chain variable regions.. Proc Natl Acad Sci USA. 1991; 88(19):8357-61. (Immunogen: Flow cytometry, Functional assay, Stimulation). View Reference
      2. Dong T, Stewart-Jones G, Chen N, et al. HIV-specific cytotoxic T cells from long-term survivors select a unique T cell receptor.. J Exp Med. 2004; 200(12):1547-57. (Clone-specific: Flow cytometry). View Reference
      3. Folch G, Jabado-Michaloud J, Lefranc M-P. Reagents monoclonal antibodies: anti-human TRBV, IMGT repertoire, IMGT Web resources. http://www.imgt.org/IMGTrepertoire/Regulation/antibodies/human/TRB/TRBV/Hu_TRBVMab.html. IMGT®, the international ImMunoGenetics informatio. 2020; 1-4. (Clone-specific).
      4. Mongkolsapaya J, Jaye A, Callan MF, Magnusen AF, McMichael AJ, Whittle HC. Antigen-specific expansion of cytotoxic T lymphocytes in acute measles virus infection.. J Virol. 1999; 73(1):67-71. (Clone-specific: Flow cytometry). View Reference
      5. Moss P, Gillespie G, Frodsham P, Bell J, Reyburn H. Clonal populations of CD4+ and CD8+ T cells in patients with multiple myeloma and paraproteinemia. Blood. 1996; 87(8):3296-3306. (Clone-specific: Flow cytometry). View Reference
      6. Salameire D, Solly F, Fabre B, et al. Accurate detection of the tumor clone in peripheral T-cell lymphoma biopsies by flow cytometric analysis of TCR-Vβ repertoire.. Mod Pathol. 2012; 25(9):1246-57. (Clone-specific: Flow cytometry). View Reference
      View All (6) View Less
      568086 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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