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      Alexa Fluor™ 647 Mouse Anti-Human CD138
      Alexa Fluor™ 647 Mouse Anti-Human CD138
      Multiparameter flow cytometric analysis of CD138 expression on viable Human U266 myeloma cells. Cells from the Human U266 (Myeloma, ATCC TIB-196™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and stained with either Alexa Fluor™ A647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dotted line histogram) or Alexa Fluor™ A647 Mouse Anti-CD138 antibody (Cat. No. 570743/570744; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CD138 expression (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (DAPI-negative) U266 cells. Flow cytometry and data analysis were performed using a BD Celesta™ Cell Analyzer System and FlowJo™ Software.
      Alexa Fluor™ 647 Mouse Anti-Human CD138
      Multiparameter flow cytometric analysis of CD138 expression on viable Human lymphocytes. Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block™ (Cat. No. 564220). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD38 (Cat. No. 562444) and Alexa Fluor™ A647 Mouse Anti-Human CD138 (Cat. No. 564219/564220) antibodies. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD138 versus CD38 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD Celesta™ Cell Analyzer System and FlowJo™ software.
      Alexa Fluor™ 647 Mouse Anti-Human CD138 Alexa Fluor™ 647 Mouse Anti-Human CD138
      Multiparameter flow cytometric analysis of CD138 expression on viable Human U266 myeloma cells. Cells from the Human U266 (Myeloma, ATCC TIB-196™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and stained with either Alexa Fluor™ A647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dotted line histogram) or Alexa Fluor™ A647 Mouse Anti-CD138 antibody (Cat. No. 570743/570744; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CD138 expression (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (DAPI-negative) U266 cells. Flow cytometry and data analysis were performed using a BD Celesta™ Cell Analyzer System and FlowJo™ Software.
      Multiparameter flow cytometric analysis of CD138 expression on viable Human lymphocytes. Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block™ (Cat. No. 564220). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD38 (Cat. No. 562444) and Alexa Fluor™ A647 Mouse Anti-Human CD138 (Cat. No. 564219/564220) antibodies. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD138 versus CD38 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD Celesta™ Cell Analyzer System and FlowJo™ software.
      Product Details
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      BD Pharmingen™
      SDC1; Syndecan 1; Syndecan-1
      Human (QC Testing)
      Mouse BALB/c IgG1
      Human U266 Myeloma Cell Line
      Flow cytometry (Routinely Tested)
      5 µl/test
      6382
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. An isotype control should be used at the same concentration as the antibody of interest.
      8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      570743 Rev. 1
      Antibody Details
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      B-A38

      The B-A38 monoclonal antibody specifically recognizes an extracellular epitope on human CD138 that is also known as Syndecan-1 (SDC-1). CD138 is a transmembrane type I and surface heparan sulfate proteoglycan that is expressed as an ~65-70 kDa core protein dimer with variable glycosylation. CD138 is encoded by SDC1 (syndecan 1) which belongs to the syndecan proteoglycan family. CD138 is expressed on pre-B cells, immature B cells, and plasma cells, but not on mature circulating B-lymphocytes, as well as by embryonic mesenchymal cells and certain epithelial and endothelial cells. It is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. CD138 is involved in many cellular functions including cell-cell and cell matrix adhesion. It binds to multiple extracellular matrix (ECM) proteins and soluble bioactive factors through its heparan sulfate side chains. ECM protein ligands for CD138 include fibronectin, certain collagens, tenascin, thrombospondin, and antithrombin III. Certain cytokines and growth factors are bound by CD138 and accumulated on CD138-positive cells which contributes to the sensitive recognition and response to these factors by neighboring cells. These may include fibroblast, hepatocyte, and epidermal growth factors and April. A soluble shed form of CD138 has also been described which can affect the stability and function of CD138.

      570743 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      570743 Rev.1
      Citations & References
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      View product citations for antibody "570743" on CiteAb

      Development References (6)

      1. Couchman JR. Syndecans: proteoglycan regulators of cell-surface microdomains?. Nat Rev Mol Cell Biol. 2003; 4(12):926-37. (Biology). View Reference
      2. Kuchen S, Robbins R, Sims GP, et al. Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration.. J Immunol. 2007; 179(9):5886-96. (Clone-specific: Flow cytometry). View Reference
      3. Lisenko K, Schönland S, Hegenbart U, et al. Potential therapeutic targets in plasma cell disorders: A flow cytometry study.. Cytometry B Clin Cytom. 2017; 92(2):145-152. (Clone-specific: Flow cytometry). View Reference
      4. Sanderson RD, Yang Y. Syndecan-1: a dynamic regulator of the myeloma microenvironment.. Clin Exp Metastasis. 2008; 25(2):149-59. (Biology). View Reference
      5. Warford A, Flack G, Conquer JS, Zola H, McCafferty J. Assessing the potential of immunohistochemistry for systematic gene expression profiling.. J Immunol Methods. 2007; 318(1-2):125-37. (Clone-specific: Immunohistochemistry). View Reference
      6. Yang Y, MacLeod V, Dai Y, et al. The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy.. Blood. 2007; 110(6):2041-8. (Clone-specific: Flow cytometry). View Reference
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      570743 Rev. 1

       

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.