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Alexa Fluor® 488 Rat Anti-Mouse CD127
Alexa Fluor® 488 Rat Anti-Mouse CD127

Flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.

     Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster anti-Mouse CD3e antibody (Cat.No. 553066) and either Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (Cat. No. 557726, dashed line histogram) or with the Alexa Fluor® 488 Rat anti-Mouse CD127 antibody (Cat. No. 561533, solid line histogram).  Histograms were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.

     Middle and Right Panels: Splenocytes from BALB/c mice were stained with PE Hamster anti-Mouse CD3e antibody (Cat.No. 553064/561824/553063) and with either Alexa Fluor® 488 Rat IgG2b, κ isotype control (middle panel) or the Alexa Fluor® 488 Rat anti-Mouse CD127 antibody (right panel).  Dot plots were derived from gated events based on light scattering characteristics for viable spleen cells.  Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.

     Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster anti-Mouse CD3e antibody (Cat.No. 553066) and either Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (Cat. No. 557726, dashed line histogram) or with the Alexa Fluor® 488 Rat anti-Mouse CD127 antibody (Cat. No. 561533, solid line histogram).  Histograms were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.

     Middle and Right Panels: Splenocytes from BALB/c mice were stained with PE Hamster anti-Mouse CD3e antibody (Cat.No. 553064/561824/553063) and with either Alexa Fluor® 488 Rat IgG2b, κ isotype control (middle panel) or the Alexa Fluor® 488 Rat anti-Mouse CD127 antibody (right panel).  Dot plots were derived from gated events based on light scattering characteristics for viable spleen cells.  Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Product Details
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BD Pharmingen™
Interleukin-7 receptor alpha chain; IL-7R alpha; IL-7RA; IL-7Rα; Il7r
Mouse (QC Testing)
Rat IgG2b, κ
BALB/c mouse pre-B cell line 1A9
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10892634
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561533 Rev. 2
Antibody Details
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SB/199

The SB/199 monoclonal antibody specifically binds to mouse CD127 which is also known as, the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological effects of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR expression upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR+ T cells.

    

561533 Rev. 2
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
561533 Rev.2
Citations & References
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Development References (10)

  1. Akashi K, Kondo M, Weissman IL. Role of interleukin-7 in T-cell development from hematopoietic stem cells. Immunol Rev. 1998; 165:13-28. (Biology). View Reference
  2. Brugnera E, Bhandoola A, Cibotti R, et al. Coreceptor reversal in the thymus: signaled CD4+8+ thymocytes initially terminate CD8 transcription even when differentiating into CD8+ T cells. Immunity. 2000; 13(1):59-71. (Biology). View Reference
  3. Faust EA, Saffran DC, Toksoz D, Williams DA, Witte ON. Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells. J Exp Med. 1993; 177(4):915-923. (Biology). View Reference
  4. Fujihashi K, Kawabata S, Hiroi T, et al. Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 1996; 93(8):3613-3618. (Biology). View Reference
  5. Goodwin RG, Friend D, Ziegler SF et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell. 1990; 60(6):941-951. (Biology). View Reference
  6. Henderson AJ, Narayanan R, Collins L, Dorshkind K. Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells. J Immunol. 1992; 149(6):1973-1979. (Biology). View Reference
  7. Kouro T, Medina KL, Oritani K, Kincade PW. Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators. Blood. 2001; 97(9):2708-2715. (Biology). View Reference
  8. Noguchi M, Nakamura Y, Russell SM, et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. Science. 1993; 262(5141):1877-1880. (Biology). View Reference
  9. Peschon JJ, Morrissey PJ, Grabstein KH, et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med. 1994; 180(5):1955-1960. (Biology). View Reference
  10. Yamashita Y, Oritani K, Miyoshi EK, Wall R, Bernfield M, Kincade PW. Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol. 1999; 162(10):5940-5948. (Clone-specific). View Reference
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561533 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.