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Alexa Fluor™ 488 Mouse Anti-Human CD271
Alexa Fluor™ 488 Mouse Anti-Human CD271

Flow cytometric analysis of CD271 expression on SK-N-MC cells. Cells from the human SK-N-MC neuroblastoma cell line (ATCC HTB-10) were stained with either Alexa Fluor™ 488 Mouse Anti-Human CD271 antibody (Cat. No. 567393/567394; solid line histogram) or Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; dashed line histogram). Flow cytometric fluorescence histograms showing CD271 expression (or Ig Isotype staining) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Flow cytometric analysis of CD271 expression on SK-N-MC cells. Cells from the human SK-N-MC neuroblastoma cell line (ATCC HTB-10) were stained with either Alexa Fluor™ 488 Mouse Anti-Human CD271 antibody (Cat. No. 567393/567394; solid line histogram) or Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; dashed line histogram). Flow cytometric fluorescence histograms showing CD271 expression (or Ig Isotype staining) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Product Details
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BD Pharmingen™
NGFR; NGF Receptor; TNFRSF16
Human (QC Testing)
Mouse IgG1, κ
Human NGFR Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
VIII 80150
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  6. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
567393 Rev. 1
Antibody Details
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C40-1457

The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR). CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves. It is also expressed by some epithelial, mesenchymal and lymphoid tissues. NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system. NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system. NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]. High affinity binding of NGF requires that both receptor molecules be expressed. NGFR is expressed on human and rat lymphocytes. A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271. It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation. NGF may also function as a B-cell growth factor.

567393 Rev. 1
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
567393 Rev.1
Citations & References
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Development References (7)

  1. Brodie C, Gelfand EW. Functional nerve growth factor receptors on human B lymphocytes. Interaction with IL-2. J Immunol. 1992; 148(11):3492-3497. (Biology). View Reference
  2. Chesa PG, Rettig WJ, Thomson TM, Old LJ, Melamed MR. Immunohistochemical analysis of nerve growth factor receptor expression in normal and malignant human tissues. J Histochem Cytochem. 1988; 36(4):383-389. (Biology). View Reference
  3. Hempstead BL, Martin-Zanca D, Kaplan DR, Parada LF, Chao MV. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature. 1991; 350(6320):678-683. (Biology). View Reference
  4. Holling TM, Bergevoet MW, Wilson L, et al. A Role for EZH2 in Silencing of IFN-γ Inducible MHC2TA Transcription in Uveal Melanoma. J Immunol. 2007; 179(8):5317-5325. (Biology). View Reference
  5. Kanellopoulou C, Muljo SA, Dimitrov SD, et al. X chromosome inactivation in the absence of Dicer.. Proc Natl Acad Sci U S A. 2009; 106(4):1122-1127. (Biology). View Reference
  6. Thompson SJ, Schatteman GC, Gown AM, Bothwell M. A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989; 92(4):415-423. (Biology). View Reference
  7. Van Landuyt K, Lories R, Jones E, et al. Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis. Arthritis Res Ther. 2001; 12(1):R15. (Biology: Flow cytometry). View Reference
View All (7) View Less
567393 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.