Skip to main content Skip to navigation
Pacific Blue™ Mouse IgG1, κ Isotype Control
Pacific Blue™ Mouse IgG1, κ Isotype Control

Flow cytometry analysis of Pacific Blue™ Mouse IgG1, ĸ Isotype Control on human lymphocytes. Whole blood was stained with Pacific Blue™ Mouse IgG1, ĸ Isotype Control (Cat. No. 558120, solid line) or left unstained (dotted line). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The flourescence histogram showing Ig isotype control staining (or unstained cells) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

Flow cytometry analysis of Pacific Blue™ Mouse IgG1, ĸ Isotype Control on human lymphocytes. Whole blood was stained with Pacific Blue™ Mouse IgG1, ĸ Isotype Control (Cat. No. 558120, solid line) or left unstained (dotted line). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The flourescence histogram showing Ig isotype control staining (or unstained cells) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
IgG1, kappa; Ighg1, Igh-4, γg1; Igk-C, IGKC, Ig κ
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry, Isotype control (Routinely Tested)
0.2 mg/ml
AB_397040
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody is conjugated to Pacific Blue™ under optimum conditions, and unreacted Pacific Blue™ was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Pacific Blue™ has a maximum absorption of 416 nm and maximum emission of 451 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558120 Rev. 6
Antibody Details
Down Arrow Up Arrow
MOPC-21

The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

558120 Rev. 6
Format Details
Down Arrow Up Arrow
Pacific Blue
Pacific Blue™ is part of the BD violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 404-nm and an emission maximum (Em Max) at 455-nm. Pacific Blue is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
Pacific Blue
Violet 405 nm
404 nm
455 nm
558120 Rev.6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.