Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
The Anti–HLA-DQ antibody, clone SK10, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with tonsillar B lymphocytes (non–E-rosetting cells). Anti-HLA-DQ (Leu-10) recognizes a class II major histocompatibility complex (MHC) antigen, Mr 26 to 34 kdaltons (kDa), distinct from HLA-DR and HLA-DP antigens. Anti-HLA-DQ recognizes a common polymorphic epitope present on HLA-DQ molecules of cells expressing DQw1 and DQw3 (usually associated with DR1, DR2, DR4, DR5, DRw8, DRw9, and DRw10) and absent from DQw2 (usually associated with DR3, and DR7). Therefore, Anti-HLA-DQ will not react with cells from DR3 or DR 7 homozygotes or DR3/7 heterozygotes.
Development References (8)
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Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.. Immunogenetics. 1984; 19(3):179-94. (Biology). View Reference
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Chen YX, Evans RL, Pollack MS, et al. Characterization and expression of the HLA-DC antigens defined by anti-Leu-10. Hum Immunol. 1984; 10:221-235. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Crumpton MF, Bodmer JG, Bodmer WF, Heyes JM, Lindsay J, Rudd CE. Biochemistry of class II antigens: workshop report. In: :29.
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Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). View Reference
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Gonwa TH, Frost JP, Karr RW. All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-γ. J Immunol. 1986; 137:519-524. (Biology).
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Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). View Reference
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