FITC Mouse Anti-Human CD11a

BD™ FITC Mouse Anti-Human CD11a

Name: FITC Mouse Anti-Human CD11a
Brand: BD™
SKU: 347983

Clone G25.2 (also known as G-25.2)

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: ITGAL; LFA-1 alpha; LFA-1A; LFA1A; integrin alpha L

Reactivity: Human

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Human Cytotoxic T Lymphocytes

Application: Flow cytometry

Concentration: 6.25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V S167

RRID: AB_400366

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The monoclonal antibody is supplied as 25 µg purified immunoglobulin in 2.0 mL (12.5 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 12.5 µg in 2.0 mL (6.25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

G25.2

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Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

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Citations & References

Development References (7)

Anderson DC, Springer TA. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.. Annu Rev Med. 1987; 38:175-94. (Biology). View Reference
Clayberger C, Wright A, Medeiros LJ, et al. Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance.. Lancet. 1987; 2(8558):533-6. (Biology). View Reference
Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.. Nature. 1989; 341(6243):619-24. (Biology). View Reference
Fischer A, Griscelli C, Blanche S, et al. Prevention of graft failure by an anti-HLFA-1 monoclonal antibody in HLA-mismatched bonemarrow transplantation. Lancet. 1986; 1058-1061. (Biology).
Inghirami G, Wieczorek R, Zhu BY, Silber R, Dalla-Favera R, Knowles DM. Differential expression of LFA-1 molecules in non-Hodgkin's lymphoma and lymphoid leukemia. Blood. 1988; 72(4):1431-1434. (Biology). View Reference
Mentzer SJ, Faller DV, Burakoff SJ. Interferon-gamma induction of LFA-1-mediated homotypic adhesion of human monocytes.. J Immunol. 1986; 137(1):108-13. (Biology). View Reference
Uciechowski P, Schmidt RE. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:543.

View All (7)

347983 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.