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Anti-Human CD15 FITC/CD117 PE

BD Oncomark™ Anti-Human CD15 FITC/CD117 PE

Product Details
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BD Oncomark™
Flow cytometry
Phosphate buffered saline with gelatin and 0.1% sodium azide.


CD15, clone MMA, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the U-937 histiocytic cell line.

CD117, clone 104D2, is derived from fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with the megakaryoctic cell line MOLM-1.

CD15 recognizes the human myelomonocytic antigen lacto-N-fucopentose III.

CD117 binds to the receptor for stem cell factor (SCF). It selectively recognizes NIH-3T3 cells transfected with human c-kit, the gene that codes for SCF-R.

Preparation And Storage

Store vials at 2–8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341126 Rev. 1
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Description Clone Isotype EntrezGene ID
CD15 FITC MMA IgM, κ 3815
CD117 (c-Kit) PE 104D2 IgG1, 3815
341126 Rev. 1
Citations & References
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Development References (21)

  1. Ashman LK, Cambareri A, Nguyen L, Bühring H-J. CD117 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:816-818.
  2. Berardi AC, Wang A, Levine JD, Lopez P, Scadden DT. Functional isolation and characterization of human hematopoietic stem cells. Science. 1995; 267:104-108. (Biology). View Reference
  3. Borowitz MJ, Guenther KL, Shults KE, Stelzer GT. Immunophenotyping of acute leukemia by flow cytometric analysis, Use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color analysis. Am J Clin Pathol. 1993; 100:534-540. (Biology).
  4. Briddell RA, Broudy VC, Bruno E, Brandt JE, Srour EF, Hoffman R. Further phenotypic characterization and isolation of human hematopoietic progenitor cells using a monoclonal antibody to the c-kit receptor. Blood. 1992; 79:3159-3167. (Biology). View Reference
  5. Broudy VC, Lin N, Zsebo KM, et al. Isolation and characterization of a monoclonal antibody that recognizes the human c-kit receptor. Blood. 1992; 79:338-346. (Biology). View Reference
  6. Cascavilla N, Musto P, D'Arena G, et al. CD117 (c-kit) is a restricted antigen of acute myeloid leukemia and characterizes early differentiative levels of M5 FAB subtype. Haematologica. 1998; 83(5):392-397. (Biology).
  7. Cohen PL, Hoyer JD, Kurtin PJ, Dewald GW, Hanson CA. Acute myeloid leukemia with minimal differentiation, A multiple parameter study. Am J Clin Pathol. 1998; 109(1):32-38. (Biology).
  8. Gunji Y, Nakamura M, Osawa H, et al. Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells. Blood. 1993; 3283-3289. (Biology).
  9. Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982 May; 23(2):172-188. (Biology). View Reference
  10. Lacombe F, Durrieu F, Briais A, et al. Flow cytometry CD45 gating for immunophenotyping of acute myeloid leukemia. Leukemia. 1997; 11:1878-1886. (Biology).
  11. Macedo A, Orfao A, Gonzalez M, et al. Immunological detection of blast cell subpopulations in acute myeloblastic leukemia at diagnosis: implications for minimal residual disease studies. Leukemia. 1995; 9:993-998. (Biology).
  12. Matsuo Y, Adachi T, Tsubota T, Imanishi J, Minowada J. Establishment and characterization of a novel megakaryoblastic cell line, MOLM-1, from a patient with chronic myelogenous leukemia. Hum Immunol. 1991; 4:261-264. (Biology). View Reference
  13. Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F. Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells. Blood. 1996; 88:1594-1607. (Biology). View Reference
  14. Rainer RO, Hodges L, Seltzer GT. CD 45 gating correlates with bone marrow differential. Cytometry. 1995; 22(2):139-145. (Biology).
  15. Schwartz S, Heinecke A, Zimmermann M, et al. Expression of the C-kit receptor (CD117) is a feature of almost all subtypes of de novo acute myeloblastic leukemia (AML), including cytogenetically good-risk AML, and lacks prognostic signicance. Leuk Lymphoma. 1999; 34(1-2):85-94. (Biology).
  16. Simmons P, Aylett G, Niutta S, To L, Juttner C, Ashman L. c-kit is expressed by primitive human hematopoietic cells that give rise to colony-forming cells in stroma-dependent or cytokinesupplemented culture. Exp Hematol. 1994; 22:157-165. (Biology).
  17. Skubitz K, Balke J, Ball E, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:800-805.
  18. Sun T, Sangaline R, Ryder J, et al. Gating strategy for immunophenotyping of leukemia and lymphoma. Am J Clin Pathol. 1997; 108(2):152-157. (Biology).
  19. Terstappen LW, Safford M, Könemann S, et al. Flow cytometric characterization of acute myeloid leukemia. Part II. Phenotypic heterogeneity at diagnosis.. Leukemia. 1992; 6(1):70-80. (Biology). View Reference
  20. Wells SJ, Bray RA, Stempora LL, Farhi DC. CD117/CD34 expression in leukemic blasts. Am J Clin Pathol. 1996; 106(2):192-195. (Biology).
  21. Yarden Y, Kuang W-J, Yang-Feng T, et al. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidenti ed ligand. EMBO J. 1987; 6:3341-3351. (Biology).
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341126 Rev. 1

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