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Purified Mouse Anti-Hsp110
Purified Mouse Anti-Hsp110
Western blot analysis of Hsp110 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-Hsp110 antibody.
Purified Mouse Anti-Hsp110

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of Hsp110 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-Hsp110 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Heat Shock Protein-110
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Hamster Hsp110 aa. 703-858
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
110 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610510 Rev. 1
Antibody Details
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21/Hsp110

With the exception of Hsp110, the stress or heat shock proteins have been thoroughly characterized. Hsp110 has been reported to be expressed in most tissues. Like other stress proteins, Hsp110 is induced by heat shock and its induction is associated with thermotolerance. The sequence of Hsp110 reportedly bears similarity to several Hsp70-related proteins that have been termed Hsp110/SSE. The carboxy-terminal domains of these proteins and Hsp110 contain a putative peptide-binding site. These proteins also contain six highly conserved regions found in the same progression, as well as five conserved ATP-binding motifs.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610510 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610510 Rev.1
Citations & References
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Development References (2)

  1. Lee-Yoon D, Easton D, Murawski M, Burd R, Subjeck JR. Identification of a major subfamily of large hsp70-like proteins through the cloning of the mammalian 110-kDa heat shock protein. J Biol Chem. 1995; 270(26):15725-15733. (Biology). View Reference
  2. Moore JK, Scheinman RI, Bellgrau D. The identification of a novel T cell activation state controlled by a diabetogenic gene. J Immunol. 2001; 166(1):241-248. (Biology: Western blot). View Reference
610510 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.