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Purified Mouse Anti-Gαq
Purified Mouse Anti-Gαq

Western blot analysis of Gαq on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Gαq antibody.

Purified Mouse Anti-Gαq

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721) treated with NGF. PC12 cells were serum starved for 1 hour and then stimulated with 100 ng/mL NGF for 10 minutes at 37°C.

Western blot analysis of Gαq on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Gαq antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721) treated with NGF. PC12 cells were serum starved for 1 hour and then stimulated with 100 ng/mL NGF for 10 minutes at 37°C.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Gαq aa. 22-31
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
42 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612704 Rev. 1
Antibody Details
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10/GAQ

The GTP binding regulatory proteins (G proteins) consist of three subunits: α, β, and γ. These heterotrimeric proteins function at membranes to relay signals from cell surface receptors to intracellular effectors. The α subunit is unique for each G protein and contains the site of GTP binding and hydrolysis, as well sites for receptor and effector interactions. The βγ subunit complex interacts directly with receptors and the α subunit. The Gα family includes four families: the Gαs family including Gαs , Gαo1f, and Gαt, the Gαi family including Gαi, Gαo, and Gαz, the Gαq/Gα11 family and the Gα12/13 family.  The Gαq protein is 88% homologus with  Gα11 and both are widely expressed.  These G proteins activate phospholipase C proteins, which induce calcium signaling events. G protein coupled receptors (GPCRs) involved in regulating Wnt signaling activate Gαq, phospholipase Cβ, and induce calcium-dependent activation of calpain. These events promote β-catenin nuclear export and proteolysis. Gαq has also been implicated in metabotropic glutamate receptor signaling. Thus, Gαq isoforms activate phospholipase C proteins in various G-protein coupled receptor pathways.

612704 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612704 Rev.1
Citations & References
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Development References (3)

  1. Dutt P, Kjoller L, Giel M, Hall A, Toksoz D. Activated Galphaq family members induce Rho GTPase activation and Rho-dependent actin filament assembly. FEBS Lett. 2002; 531(3):565-569. (Biology). View Reference
  2. Li G, Iyengar R. Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation. Proc Natl Acad Sci U S A. 2002 ; 99(20):13254-13259. (Biology). View Reference
  3. Strathmann M, Simon MI. G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. Proc Natl Acad Sci U S A. 1990; 87(23):9113-9117. (Biology). View Reference
612704 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.