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Purified Mouse Anti-AKAP79
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human AKAP79 aa. 180-427
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
79 kDa
250 µg/ml
AB_397706
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610314 Rev. 1
Antibody Details
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22/AKAP79

The type II cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain this localization of type II PKAs, the regulatory subunit  (RII) interacts with specific RII-anchoring proteins. For instance, attachment of type II PKA to the cytoskeleton occurs through the binding of RII to microtubule-associated protein 2 (MAP2). In brain, several proteins have been identified as PKA type II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP79 is a 79kDa human RII-anchoring protein. AKAP, PKA type II, and calcineurin (PP2B) can form a tertiary complex, suggesting that both PKA and calcineurin are targeted by a common protein to subcellular sites where they regulate the phosphorylation status of key substrates.

610314 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610314 Rev.1
Citations & References
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Development References (4)

  1. Carr DW, Stofko-Hahn RE, Fraser ID, Cone RD, Scott JD. Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.. J Biol Chem. 1992; 267(24):16816-23. (Biology). View Reference
  2. Coghlan VM, Perrino BA, Howard M. Association of protein kinase A and protein phosphatase 2B with a common anchoring protein. Science. 1995; 267(5194):108-111. (Biology). View Reference
  3. Jicha GA, Weaver C, Lane E. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999; 19(17):7486-7494. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  4. Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (4) View Less
610314 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.