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FITC Mouse Anti-EGF Receptor
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human EGF Receptor aa. 1020-1046
Immunofluorescence (Routinely Tested)
250 µg/ml
AB_399653
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612554 Rev. 1
Antibody Details
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13/EGFR

Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cγ. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.

This antibody is routinely tested by immunofluorescence.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612554 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
612554 Rev.1
Citations & References
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Development References (4)

  1. Carpenter G. Receptors for epidermal growth factor and other polypeptide mitogens. Annu Rev Biochem. 1987; 56:881-914. (Biology). View Reference
  2. Golubovskaya V, Beviglia L, Xu LH, Earp HS 3rd, Craven R, Cance W. Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells. J Biol Chem. 2002; 277(41):38978-38987. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  3. Ward CW, Gough KH, Rashke M, Wan SS, Tribbick G, Wang J. . Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor.. J Biol Chem. 1996; 271(10):5603-5609. (Biology). View Reference
  4. Wu TT, Castle JD. Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor. Mol Biol Cell. 1998; 9(7):1661-1674. (Clone-specific: Immunofluorescence). View Reference
View All (4) View Less
612554 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.