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Recombinant Human MCP-3

Recombinant Human MCP-3

(RUO)
Product Details
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Description

Human monocyte chemoattractant protein-3 (MCP-3) is a member of CC chemokine family. It is produced by monocytes, platelets, fibroblasts and osteosarcoma cell line MG-63. The target cells of MCP-2 include monocytes, lymphocytes, basophils, eosinophils, NK cells, dendritic cells and neutrophils.  Recombinant human MCP-3 (MN 554629) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2.0 mg/mL bovine serum albumin, with no preservatives. Recombinant human MCP-3 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per μg of human MCP-3, as measured in a chromogenic LAL assay.

BD Pharmingen™
200 µg/ml
Human (QC Testing)
Frozen aqueous buffered solution containing BSA.
RUO
AB_2868999
ELISA Standard (Routinely Tested), Bioassay (Tested During Development)


Preparation And Storage

Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

Upon initial thawing, recombinant human MCP-3 (MN 554629) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended.  Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier protein may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.

ELISA Standard:  Recombinant human MCP-3 (MN 554629) can be useful as a quantitative standard for measuring human MCP-3 protein levels using sandwich ELISA with the purified 9H11 antibody (Cat. No. 551228) as a capture antibody and the biotinylated 6H5 antibody (Cat. No. 555034) as the detection antibody.  To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant human MCP-3 from 1600-12 pg/mL to be included for each ELISA plate.

Bioassay:  Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.  An activity range encompassing an ED50 = 10 - 400 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554629 Rev. 2
Citations & References
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Development References (4)

  1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). View Reference
  2. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). View Reference
  3. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.
  4. Van Damme J, Proost P, Lenaerts JP, Opdenakker G.. Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family. J Exp Med. 1992; 176(1):59-65. (Biology). View Reference
View All (4) View Less
554629 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.