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Purified NA/LE Hamster anti-Mouse IFN-γ
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Armenian Hamster IgG1, κ
Recombinant Mouse IFN-γ
ELISA (Routinely Tested), Neutralization (Tested During Development)
1.0 mg/ml
AB_396744
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557530 Rev. 3
Antibody Details
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H22

The H22 antibody reacts with mouse interferon-γ  (IFN-γ) , but reportedly not with mouse IFN-α , IFN-β , IL-6, or human IFN-γ.  Although not routinely tested, the H22 antibody has been reported to inhibit the in vitro and in vivo activities of mouse IFN-γ.  Specific binding of  I-125 labeled recombinant mouse IFN-γ to IFN-γ receptor-positive cells has been reported to be blocked by H22.  Although the H22 antibody is reported to react with rat IFN-γ , its reactivity with IFN-γ  from other species has not been tested.

557530 Rev. 3
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
557530 Rev.3
Citations & References
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Development References (5)

  1. Bancroft GJ, Schreiber RD, Bosma GC, Bosma MJ, Unanue ER. A T cell-independent mechanism of macrophage activation by interferon-gamma. J Immunol. 1987; 139(4):1104-1107. (Clone-specific: Neutralization). View Reference
  2. Buchmeier NA, Schreiber RD. Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection. Proc Natl Acad Sci U S A. 1985; 82(21):7404-7408. (Clone-specific: Neutralization). View Reference
  3. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Methodology). View Reference
  4. Leiby DA, Fortier AH, Crawford RM, Schreiber RD, Nacy CA. In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies. Infect Immun. 1992; 60(1):84-89. (Clone-specific: Neutralization). View Reference
  5. Schreiber RD, Hicks LJ, Celada A, Buchmeier NA, Gray PW. Monoclonal antibodies to murine -interferon which differentially modulate macrophage activation and antiviral activity. J Immunol. 1985; 134(3):1609-1618. (Clone-specific: Neutralization). View Reference
View All (5) View Less
557530 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.