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Purified Rat Anti-Mouse IL-9
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a
Recombinant mouse IL-9
ELISA Capture (Routinely Tested)
1.0 mg/ml
AB_394096
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: Purified D8402E8 antibody (Cat. No. 551218) is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-9 protein levels.  Purified D8402E8 antibody can be paired with the biotinylated D9302C12 antibody as the detecting antibody (Cat. No. 554473), with recombinant mouse IL-9 as the standard. Purified D8402E8 antibody should be titrated 2.0 - 6.0 µg/ml to determine optimal concentration for ELISA capture. For maximal sensitivity, Pharmingen recommends an overnight incubation (4°C) of samples/standards with the coated capture antibody. For specific methodology, please visit our web site, www.bdbiosciences.com , and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

        This ELISA pair shows no cross-reactivity with any of the cytokines tested ( e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL- 7, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGFAA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).

        For neutralization of mouse IL-9 bioactivity, we recommend an alternate clone, the D9302C12 antibody in the NA/LE™ format (Cat. No. 554472).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551218 Rev. 1
Antibody Details
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D8402E8

The D8402E8 antibody reacts with mouse IL-9. The immunogen used to generate the D8402E8 hybridoma was HPLC-purified recombinant mouse IL-9. This is a non-neutralizing antibody.

551218 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
551218 Rev.1
Citations & References
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Development References (2)

  1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
  2. Renauld JC, Kermouni A, Vink A, Louahed J, Van Snick J. Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis. J Leukoc Biol. 1995; 57(3):353-360. (Clone-specific). View Reference
551218 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.