BD Horizon™ RB780 Mouse Anti-Human Somatostatin
Clone U24-354 (RUO)
Flow cytometric analysis of Somatostatin expression in Human Somatostatin-transfected 293F cells. Human 293F untransfected (dashed line histogram) and somatostatin-transfected (solid line histogram) cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and stained with BD Horizon™ RB780 Mouse Anti-Human Somatostatin (Cat. No. 570992/571063). The fluorescence histograms showing Somatostatin expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
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Flow cytometric analysis of Somatostatin expression in Human Somatostatin-transfected 293F cells. Human 293F untransfected (dashed line histogram) and somatostatin-transfected (solid line histogram) cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and stained with BD Horizon™ RB780 Mouse Anti-Human Somatostatin (Cat. No. 570992/571063). The fluorescence histograms showing Somatostatin expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
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Somatostatin is produced by neuroendocrine neurons in the hypothalamus and δ cells in the islets of Langerhans of the pancreas. It is a regulatory hormone that affects neurotransmission, cell proliferation, and numerous hormones of the endocrine system. Via interaction with high affinity G protein-coupled somatostatin receptors, it inhibits the secretion of somatotropin (also known as growth hormone or GH), thyroid-stimulating hormone (thyrotropin or TSH), and most gastrointestinal and pancreatic hormones, including glucagon and insulin. The expression of somatostatin can be used to monitor the pancreatic differentiation of pluripotent stem cells.
The human somatostatin gene, SST, encodes the precursor molecule preprosomatostatin (amino acids 1-116), which is cleaved to form prosomatostatin (amino acids 15-116), which in turn is cleaved to form either of 2 alternative active somatostatin peptides, somatostatin-28 (amino acids 89-116) or somatostatin-14 (amino acids 103-116). The U16-850 monoclonal antibody detects human preprosomatostatin in somatostatin-producing cells.
Development References (4)
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D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology). View Reference
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Goodman RH, Montminy MR, Low MJ, Habener JF. Biosynthesis of rat preprosomatostatin.. Adv Exp Med Biol. 1985; 188:31-47. (Biology). View Reference
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Kelly OG, Chan MY, Martinson LA, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011; 29(8):750-756. (Biology). View Reference
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Montminy MR, Goodman RH, Horovitch SJ, Habener JF. Primary structure of the gene encoding rat preprosomatostatin.. Proc Natl Acad Sci USA. 1984; 81(11):3337-40. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.