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Purified Rat Anti-Mouse CD98
Product Details
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BD Pharmingen™
Cd98; CD98 heavy chain; Slc3a2; 4F2hc; 4F2; Ly-10; Ly-m10; Ly10; Mdu1
Mouse (QC Testing)
Rat IgG2a, κ
Not Reported
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
17254
AB_2190819
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

This clone H202-141 had been tested in house during development by immunohistochemical staining of acetone-fixed frozen mouse thymus and spleen tissue sections with antibody concentration of 1-10 µg/ml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557479 Rev. 12
Antibody Details
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H202-141

The H202-141 monoclonal antibody specifically reacts with CD98 (4F2, Ly-10), a heterodimer composed of an 80-kDa glycosolated heavy chain covalently linked to a 34-kDa light chain, which is reported to be involved in amino acid transport.  H202-141 mAb detects high levels of antigen expression on activated lymphocytes and established cell lines and lower levels of expression on resting leukocytes.

557479 Rev. 12
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
557479 Rev.12
Citations & References
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Development References (2)

  1. Chan MM, Hammerling U, Stutman O. The Ly-10 antigen is a marker of mouse-activated T lymphocytes. Immunogenetics. 1988; 28(6):425-432. (Biology). View Reference
  2. Nakamura E, Sato M, Yang H, et al. 4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer. J Biol Chem. 1999; 274(5):3009-3016. (Biology). View Reference
557479 Rev. 12

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.