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      Purified NA/LE Mouse Anti-Human CD93
      Purified NA/LE Mouse Anti-Human CD93
      Expression of CD93 by unstimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stained with the purified NA/LE Mouse Anti-Human CD93 antibody (Cat. No. 552954, shaded histogram), or Purified NA/LE Mouse IgG2b, κ Isotype Control (Cat. No. 559530, open histogram).  Secondary staining was carried out with Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD93 (or Ig Isotype Control) expression were derived from gated events with the side and forward light-scatter characteristics of viable PBMCs.
      Purified NA/LE Mouse Anti-Human CD93
      Expression of CD93 by unstimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stained with the purified NA/LE Mouse Anti-Human CD93 antibody (Cat. No. 552954, shaded histogram), or Purified NA/LE Mouse IgG2b, κ Isotype Control (Cat. No. 559530, open histogram).  Secondary staining was carried out with Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD93 (or Ig Isotype Control) expression were derived from gated events with the side and forward light-scatter characteristics of viable PBMCs.
      Product Details
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      BD Pharmingen™
      C1QR1; C1qRP; C1qR(P); C1q/MBL/SPA Receptor; MXRA4; GR11; Dj737e23.1
      Human (QC Testing)
      Mouse BALB/c IgG2b, κ
      Clq-CLF-binding proteins
      Flow cytometry (Routinely Tested), Neutralization (Reported)
      1.0 mg/ml
      22918
      AB_394531
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      552954 Rev. 3
      Antibody Details
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      R139

      The R139 monoclonal antibody specifically binds to CD93 which is also known as Complement component C1q receptor (C1qR), C1q receptor 1 (C1qR1), or Matrix-remodeling-associated protein 4 (MXRA4). The immunogen used to generate the R139 hybridoma was a preparation of CD93 protein. Human CD93 is a transmembrane glycoprotein that is highly expressed on monocytes, macrophages, granulocytes, and endothelial cells but not on T and B lymphocytes. CD93 is also known as the C1q/MBL/SPA Receptor as it binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R139 is suitable to detect CD93 expression on cells of myeloid lineage by flow cytometry, and CD93 in cellular lysates by Western blotting or immunoprecipitation. In addition, R139 reportedly neutralizes C1q-mediated enhancement of phagocytosis. CD93 has also been reported to define a human stem cell population with hematopoietic and hepatic potential.

      552954 Rev. 3
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      552954 Rev.3
      Citations & References
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      Development References (8)

      1. Danet GH, Luongo JL, Butler G, et al. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential.. Proc Natl Acad Sci USA. 2002; 99(16):10441-5. (Biology). View Reference
      2. Guan E, Robinson SL, Goodman EB, Tenner AJ. Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis. J Immunol. 1994; 152(8):4005-4016. (Immunogen). View Reference
      3. Guan EN, Burgess WH, Robinson SL, Goodman EB, McTigue KJ, Tenner AJ. Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis. J Biol Chem. 1991; 266(30):20345-20355. (Immunogen). View Reference
      4. Nepomuceno RR, Henschen-Edman AH, Burgess WH, Tenner AJ. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. Immunity. 1997; 6(2):119-129. (Clone-specific). View Reference
      5. Nepomuceno RR, Ruiz S, Park M, Tenner AJ. C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity. J Immunol. 1999; 162(6):3583-3589. (Clone-specific). View Reference
      6. Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998; 160(4):1929-1935. (Clone-specific). View Reference
      7. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). View Reference
      8. Tenner AJ. C1q receptors: regulating specific functions of phagocytic cells. Immunobiology. 1998; 199(2):250-264. (Biology). View Reference
      View All (8) View Less
      552954 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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