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Purified Mouse Anti-Human MRP1
Purified Mouse Anti-Human MRP1
Profile of human MRP1 (clone QCRL-3) reactivity on fixed, permeabilized H69AR cell line analyzed by flow cytometry. The cell line was fixed and permeabilized with BD Cytofix/Cytoperm™  Cat. No. 554714. Second step staining with Cat. No. 555988.
Profile of human MRP1 (clone QCRL-3) reactivity on fixed, permeabilized H69AR cell line analyzed by flow cytometry. The cell line was fixed and permeabilized with BD Cytofix/Cytoperm™  Cat. No. 554714. Second step staining with Cat. No. 555988.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2a, κ
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
AB_2275850
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557594 Rev. 3
Antibody Details
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QCRL-3

Reacts with an intracellular epitope of the multidrug resistance protein (MRP1). MRP1 is a 190 kDa integral membrane phosphoglycoprotein, member of the ATP-binding cassette transporter proteins, overexpressed in some drug-selected resistant cell lines and has been shown to cause multidrug resistance in transfected cells. Clone QCRL-3 was generated using non-denatured membranes from H69AR, an MRP1-overexpressing, multidrug resistant, drug-selected cell line. Its epitope has been localized to the first nucleotide binding domain of MRP1 between amino acids 617 and 932.3 QCRL-3 does not cross-react with human MDR1 or MDR3 gene products, nor with murine MRP1. It is reported that mAb QCRL-3 inhibits the ATP-dependent transport activity of MRP1 in inside-out membrane vesicles.

557594 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
557594 Rev.3
Citations & References
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Development References (4)

  1. Cole SP, Bhardwaj G, Gerlach JH, et al. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line. Science. 1992; 258(5088):1650-1654. (Biology). View Reference
  2. Hipfner DR, Gauldie SD, Deeley RG, Cole SP. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 1994; 54(22):5788-5792. (Biology). View Reference
  3. Hipfner DR, Mao Q, Qiu W, et al. Monoclonal antibodies that inhibit the transport function of the 190-kDa multidrug resistance protein, MRP. Localization of their epitopes to the nucleotide-binding domains of the protein. J Biol Chem. 1999; 274(22):15420-15426. (Biology). View Reference
  4. Loe DW, Almquist KC, Deeley RG, Cole SP. Multidrug resistance protein (MRP)-mediated transport of leukotriene C4 and chemotherapeutic agents in membrane vesicles. Demonstration of glutathione-dependent vincristine transport. J Biol Chem. 1996; 271(16):9675-9682. (Biology). View Reference
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557594 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.