Skip to main content Skip to navigation
PE Rat Anti-Mouse Vβ 10[b] TCR
PE Rat Anti-Mouse Vβ 10[b] TCR

Two-color analysis of the expression of Vβ 10[b] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Rat Anti-Mouse Vβ 10[b] TCR (Cat. No. 553285), FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047), and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) monoclonal antibodies. The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

Two-color analysis of the expression of Vβ 10[b] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Rat Anti-Mouse Vβ 10[b] TCR (Cat. No. 553285), FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047), and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) monoclonal antibodies. The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
TCR Vβ10; TCR V beta 10; TCR Vb10
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
Mouse Cytotoxic T-Cell Clone Soluble αβ TCR
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394757
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells. PE has been reported to bind mouse B cells via FcγII receptors. For immunostaining of mouse cells, this binding can be prevented by preincubating cells with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553285 Rev. 2
Antibody Details
Down Arrow Up Arrow
B21.5

The B21.5 monoclonal antibody specifically recognizes the Vβ 10 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. It does not react with mice having the a (eg, C57BR, C57L, SJL, SWR) or c (eg, RIII) Tcrb haplotype.

553285 Rev. 2
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
553285 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (2)

  1. Necker A, Rebai N, Matthes M, et al. Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells. Eur J Immunol. 1991; 21(12):3035-3040. (Immunogen). View Reference
  2. Takizawa F, Kinet JP, Adamczewski M. Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G. J Immunol Methods. 1993; 162(2):269-272. (Biology). View Reference
553285 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.