PE Mouse Anti-Mouse H-2D[b]

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753215] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ PE Mouse Anti-Mouse H-2D[b]

Name: PE Mouse Anti-Mouse H-2D[b]
Brand: BD Pharmingen™
SKU: 553574

Clone KH95

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Histocompatibility-2Db; H-2Db

Reactivity: Mouse (QC Testing)

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: C57BL/10 mouse skin graft and splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_394932

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG2b, κ Isotype Control RUO

Size 0.1 mg Cat No. 559529

553574 Rev. 10

Antibody Details

KH95

The KH95 antibody specifically recognizes the H-2D[b] MHC class I alloantigen. Reactivity with other haplotypes (e.g., a, d, f, k, n, p, q, r, s, u, v) has not been observed.

553574 Rev. 10

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

553574 Rev.10

Citations & References

Development References (1)

Hasenkrug KJ, Cory JM, Stimpfling JH. Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region. Immunogenetics. 1987; 25(2):136-139. (Immunogen). View Reference

553574 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.