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PE Mouse Anti-Human Nectin-1 (CD111)
PE Mouse Anti-Human Nectin-1 (CD111)

Flow cytometric analysis of Nectin-1 (CD111) expression on TF-1 cells. Cells from the human TF-1 (Human erythroleukemia, ATCC Cat. No. CRL-2003) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Nectin-1 (CD111) antibody (Cat. No. 565766; solid line histogram).  The fluorescence histogram showing Nectin-1 (CD111) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Flow cytometric analysis of Nectin-1 (CD111) expression on TF-1 cells. Cells from the human TF-1 (Human erythroleukemia, ATCC Cat. No. CRL-2003) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Nectin-1 (CD111) antibody (Cat. No. 565766; solid line histogram).  The fluorescence histogram showing Nectin-1 (CD111) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
Nectin1; PRR1; PVRL1; PVRR1; HveC; HIgR; CLPED1; ED4; SK-12; OFC7
Human (QC Testing)
Mouse IgG1, κ
Human Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2739344
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565766 Rev. 3
Antibody Details
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CK41

The CK41 monoclonal antibody specifically binds to Nectin-1, which is also known as, CD111, Poliovirus receptor-related protein 1(PRR1, PVRL1), Herpes virus entry mediator C (HveC), or Herpesvirus Ig-like receptor (HIgR). Nectin-1 is a type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD111 is expressed on a variety of cell types including, epithelial cells, endothelial cells, neuronal cells, megakaryocytes, and CD34 positive stem cells. CD111 functions as a receptor for herpes simplex virus 1 (HSV 1) and HSV 2.  Nectin-1 also serves as an intercellular adhesion molecule helping to form junctions between cells, eg, between endothelial cells or epithelial cells. Nectin-1 can bind to other Nectin-1 molecules and Nectin family members and to CD155 (Poliovirus Receptor/PVR).

        

565766 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565766 Rev.3
Citations & References
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Development References (4)

  1. Krummenacher C, Baribaud I, Eisenberg RJ, Cohen GH. Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection. J Virol. 2003; 77(16):8985-8999. (Clone-specific: Immunofluorescence). View Reference
  2. Krummenacher C1, Baribaud I, Ponce de Leon M, et al. Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies. J Virol. 2000; 74(23):10863-10872. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation, Inhibition). View Reference
  3. Richart SM1, Simpson SA, Krummenacher C, et al. Entry of herpes simplex virus type 1 into primary sensory neurons in vitro is mediated by Nectin-1/HveC. J Virol. 2003; 77(5):3307-3311. (Clone-specific: Blocking, Inhibition). View Reference
  4. Simpson SA, Manchak MD, Hager EJ, et al. Nectin-1/HveC Mediates herpes simplex virus type 1 entry into primary human sensory neurons and fibroblasts. 2005; 11(2):208-218. (Clone-specific: Blocking, Inhibition). View Reference
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565766 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.