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FITC Mouse Anti-Human CD48
Product Details
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BD Pharmingen™
BCM1; BLAST; BLAST1; Hulym3; MEM-102; OX45; SLAMF2; TCT.1
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
20 µl
IV N68
AB_396100
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
555759 Rev. 5
Antibody Details
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TÜ145

The TÜ145 monoclonal antibody specifically binds to CD48. CD48 belongs to the CD2 family within the immunoglobulin superfamily. CD48 is a ~43 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD48 is strongly expressed on lymphocytes, monocytes and macrophages and weakly on granulocytes. The expression of CD48 is upregulated following lymphocyte activation and by Epstein-Barr virus (EBV) infection. CD48 binds with high affinity to CD244 and with low affinity to CD2. These receptor-ligand interactions can augment or inhibit leukocyte responses.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555759 Rev. 5
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
555759 Rev.5
Citations & References
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Development References (3)

  1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  2. Sandrin MS, Mouhtouris E, Vaughan HA, Warren HS, Parish CR. CD48 is a low affinity ligand for human CD2. J Immunol. 1993; 151(9):4606-4613. (Biology). View Reference
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
555759 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.