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FITC Goat Anti-Mouse Ig

FITC Goat Anti-Mouse Ig

Clone Polyclonal

(RUO)
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Goat Ig
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.5 mg/ml
AB_395197
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

This antibody was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with rat, human, bovine, and horse serum proteins (multiple adsorption).

Recommended Assay Procedures

Flow cytometry:  As a second step reagent for staining rat splenocytes, it is reactive with mouse antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. However it has been reported that reactivity with some mouse IgM monoclonal antibodies is weak. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with no non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse antibodies, particularly mouse IgG. For optimal staining of human leukocytes, with mouse IgG and IgM primary antibodies, FITC goat anti-mouse IgG + IgM (human-, bovine-, and horse-adsorbed, Cat. No. 555988) is recommended.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554001 Rev. 15
Antibody Details
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Poly1270
554001 Rev. 15
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
554001 Rev.15

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.